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campbell-lab.bsky.social
Professor in the Department of Chemistry, School of Science, The University of Tokyo. Editor-in-Chief of Protein Engineering, Design & Selection (PEDS). Opinions are my own. https://campbell.chem.s.u-tokyo.ac.jp
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Protein Engineering, Design & Selection (PEDS) invites contributions to a Special Collection titled, “Non-Canonical Amino Acids", with guest editors Prof. Huiwang Ai (Virginia) and Prof. Peng Chen (Peking). Send us your best work! academic.oup.com/peds/pages/c...

submitted 23 days ago • 0 comments

New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...

submitted 49 days ago • 1 comment

I am delighted to share that I have taken on the role of Editor-in-Chief of Protein Engineering, Design & Selection. From the dawn of molecular biology to the current golden age of protein engineering, PEDS has been a cornerstone of the community. Send us your best work! academic.oup.com/peds

submitted 60 days ago • 0 comments

🚨iGluSnFR4 is finally out!🚨🧪 We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️ www.biorxiv.org/content/10.1... 🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX #Neuroscience

submitted 74 days ago • 7 comments

Today we report the directed evolution of HUH into rHUH, a sequence-specific covalent protein tag for RNA labeling. rHUH was hard to engineer: the initial HUH template had no RNA activity, so we started with RNA-DNA hybrids. 30 rounds of selection on the yeast surface were required, and 7 cycles of

submitted 100 days ago • 3 comments

First PinkyCaMP plasmids are available via @addgene.bsky.social www.addgene.org/browse/artic... Cc @finkryan.bsky.social @massecklab.bsky.social

submitted 101 days ago • 0 comments

I, along with Dr. Sarah Shaykevich, will be speaking about red and near-infrared genetically encoded Ca2+ biosensors in the European Calcium Society (ECS) webinar on Wednesday, February 26th at 5 PM CET. This webinar series is open to everyone! docs.google.com/spreadsheets...

submitted 112 days ago • 0 comments

Doctoral student Dazhou Cheng's great work on developing a chemigenetic K+ indicator (doi.org/10.1021/jacs...) was featured in the Yomiuri Shimbun daily newspaper!

submitted 134 days ago • 0 comments

PhD students and postdoctoral researchers in the UK & Ireland can apply for a JSPS short-term (1-12 month) fellowship to study in Japan. Next deadline is Jan. 20 2025. If you are interested in engineering fluorescent biosensors, feel free to reach out! www.jsps.org/latest_calls...

submitted 167 days ago • 0 comments

Congratulations to the whole team! PinkyCaMP is a giant leap forward for red GECIs. Delighted to have contributed to the protein engineering with graduate student Shosei Imai.

submitted 170 days ago • 0 comments

Ph.D. student Dazhou Cheng developed a chemigenetic K+ indicator (HaloKBP), using an rHCaMP/HaloCaMP-type design! pubs.acs.org/doi/10.1021/...

submitted 184 days ago • 2 comments

The Asian Chemical Biology Initiative (ACBI) will hold tutorials and student interviews in Hanoi on January 19, 2025. Hope to see you there! Interested students can get more information and sign up here: www.asianchembio.com/hanoi-2025-interview Application deadline is December 1, 2024.

submitted 194 days ago • 0 comments

Excited to report an improved green fluorescent extracellular L-lactate biosensor (eLACCO2.1), and a new red fluorescent intracellular L-lactate biosensor (R-iLACCO1), for multiplexed fluorescence imaging! Deep appreciation for a great team of collaborators! rdcu.be/dpB8Y

submitted 588 days ago • 0 comments