cellsandprots.bsky.social
Prof of Biochemistry & Molecular Biology at @ubc and @msl Our team focuses on proteostasis and yeast cell biofactories
❤️ ubiquitin, chaperones & proteomics
💙 outdoor, repast, electro-rock & traveling 🇨🇭🇨🇦
https://mayor51.wixsite.com/mayorlab
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4 months after biorxiv submission sounds like a smooth ride - congrats
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You almost look like Leonard on that picture ;-)
Happy birthday- enjoy the rest of the trip
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Many people contributed to this project led by @hthrbaker.bsky.social with help from @shriyakamat.bsky.social and great collaborators in @miketilapia.bsky.social and Brian Raught's labs
The illustration was created by @taniodraws.bsky.social our go-to freelance artist for summarizing our studies.
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One limitation of our study is the use of rapidly dividing cells, where the protein homeostasis network likely differs from that in differentiated cells with lower protein production. Despite our efforts, we have yet to identify the E3 ligase involved—but we’re not giving up, so stay tuned!
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In this case, the disease-associated mutation increases interaction with the DNAJA2 chaperone, which appear to be dispensable for the folding of the wild-type protein. This interaction delays the proteolysis of the mutant protein, potentially buffering its deleterious effects.
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Using BioID, we identified chaperones, including the co-chaperone DNAJA2, that interact more with the unstable thiopurine S-methyltransferase (TPMT) variant than the wild type. DNAJA2 deletion accelerates degradation of the mutated TPMT but does not affect the wild-type protein
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Many disease-associated mutations are missense mutations causing single amino acid changes that signal proteins for degradation. We surveyed 18 unstable variants from 13 cytosolic proteins and found that all but one are at least partially degraded via proteasome- and ubiquitin-dependent pathways.
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Congratulations to the whole team!
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🤔 a lot of us will have to leave. Yes I am co-author on a MPDI journal 😬 But yeah if a see a lot of these journals in a CV, it will dampen my enthusiasm for an application
We first need to better educate our trainees about the issue (e.g. does your grad program cover the topic in a course?)
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I would love to be added !
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Thanks – who doesn't love to ride good vibes?!
P.S. I'm fine, just wanted to share this hiccup since we often tend to overemphasize our successes on SM
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When I was on the bench It wasn't uncommon for experiments to fail, then fail again, and again (you probably know the feeling). A rejected grant is not that different
In most cases, it’s not the fall that matters but how we bounce back. Each trial makes us more experienced; well that’s the theory 😉
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Makes sense - btw is SIFI still a RING ligase or we need to create RING-like category
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This is amazing - congrats!!!
How do you prevent HRI degradation before the stress ? I don’t recall HRI level to go up upon stress
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That’s encouraging. It is important for us to know this is happening without revealing technical details - we need to keep trusting some aspect of the system as well as help our trainees navigate this new reality
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Along the fact data can now be AI generated (see @thatsregrettab1.bsky.social nice demo a few months ago) we need to change how we report data - I am curious to hear how publishers and editors want to tackle this
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Of course CAT (carboxy-terminal alanine and threonine) tails should have been named otherwise (they don't have much threonine in human), but they make such a great name ;-) This Ribosomal Quality Control pathway is so remarkable.
Here is the paper's link: www.cell.com/molecular-ce...
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If a tree falls in a forest and no one is around… 😉
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These 3D print are so amazing. How do you find the time!!
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Thx - I pledged allegiance a few years ago and should now qualify ;-)
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The feeling never really leaves you (or at least I have not find a way to deal with this properly, although sharing the experience helps)… now, feeling like a fake imposter is another level I have not yet reached or even considered 😮💨
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No truth is absolute - whether or not they are opinionated, a journalist should seek to provide multiple views on a topic, but it does not have to always be equal space. Maybe like spice and salt use it in moderation to avoid over seasoning