fitzpatricklab.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

An approach for coherent periodogram averaging of tilt-series data for improved contrast transfer function estimation 👉 buff.ly/hDgWZMA #cryoEM #StructuralBiology #CryoET

submitted 14 days ago • 0 comments

Structural basis of human uromodulin filament networks in uropathogen capture pubmed.ncbi.nlm.nih.gov/40345203/ #cryoEM

submitted 27 days ago • 0 comments

>20 years ago, Zuker et al. discovered the mammalian sweet taste receptors Now, an exciting collaboration between our labs reveals the #cryoEM structure of the human sweet receptor-key to sweet taste perception-online @cp-cell.bsky.social! www.sciencedirect.com/science/article/pii/S0092867425004568

submitted 30 days ago • 1 comment

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

submitted 31 days ago • 5 comments

Nature research paper: Structural basis of lipid transfer by a bridge-like lipid-transfer protein https://go.nature.com/3Gn657r

submitted 43 days ago • 0 comments

I'm excited to share our lab's newest publication on the structure and pharmacology of human GABA transporter 3 @natcomms.nature.com. Much to our surprise, GAT3 adopts an inward-open conformation in complex with the (moderately) selective antagonist SNAP-5114. 💊 🧪 #CryoEM #OpenAccess rdcu.be/ei2KY

submitted 44 days ago • 3 comments

We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples! Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵 #CryoET #TeamTomo www.biorxiv.org/content/10.1...

submitted 44 days ago • 3 comments

Thrilled to share the structure of dimerised human PINK1 docked to an endogenous translocase array on the mitochondrial surface, composed of two TOM complexes, bridged by a VDAC2 dimer! Published today in Science www.science.org/doi/10.1126/... @wehi-research.bsky.social @komanderlab.bsky.social

submitted 85 days ago • 9 comments

@science.org Cryo-EM reveals mechanisms of natural RNA multivalency | Science www.science.org/doi/10.1126/... #RNAsky #RNAbiology #Cryo-EM

submitted 85 days ago • 2 comments

Mindblowing new study by @raflynn5.bsky.social @bostonchildrens.bsky.social, cell surface RNA-binding proteins form nanoclusters with #glycoRNA and mediate cell-penetrating peptide entry into cells 👏 www.cell.com/cell/abstrac...

submitted 99 days ago • 1 comment

Happy to share this new #glycotime work with collaborator Tony Wyss-Coray, led by grad student Sophia Shi, mucin loss at the BBB is associated with aging and cognitive decline, can be reversed by restoration with with gene therapy. Aging is a mucinopathy! www.nature.com/articles/s41...

submitted 100 days ago • 2 comments

Endogenous structure of antimalarial target PfATP4 reveals new class of apicomplexan P-type ATPase modulators www.biorxiv.org/content/10.1101/2025.02.25.640208v1 #cryoEM

submitted 100 days ago • 1 comment

In @justinditrani.bsky.social final postdoc paper (with contributions from many others) we show that forming/purifying vesicles (here from M. smegmatis) allows structure det'n of endogenous membrane proteins in their native lipid bilayer 🧵 on methods & TB biology... www.pnas.org/doi/full/10....

submitted 107 days ago • 1 comment

Synaptotoxic forms of amyloid-b and a-synuclein act through a common pathway. https://www.biorxiv.org/content/10.1101/2025.02.13.638131v1

submitted 109 days ago • 0 comments

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬 The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy www.biorxiv.org/content/10.1...

submitted 116 days ago • 15 comments

New preprint from the MemTraS team ! www.biorxiv.org/content/10.1... Great collaboration with @fronzeslab.bsky.social and Vladan Lucic to determine the first cryo electron tomography images of dopamine "synapses" extracted from adult mouse brain.

submitted 116 days ago • 0 comments

New paper from our lab @naturecomms.bsky.social! We reveal the dynamics and mechanism of target DNA traversal in #CRISPR Cas12a, a conundrum in the field! nature.com/articles/s41... #compchem We thank the amazing #HPC resources of PSC #Anton2 and SDSC

submitted 118 days ago • 3 comments

Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4

submitted 120 days ago • 6 comments

It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density, which usually isn’t attainable. Lipid-CLEM brought to you by @mathilda95.bsky.social changes that. www.biorxiv.org/content/10.1...

submitted 126 days ago • 12 comments

Proud to share: "Ensemble docking for intrinsically disordered proteins" from Dartmouth undergrad Anjali Dhar 24' and grad student Tommy Sisk. We present ensemble docking strategies for IDPs that, remarkably, seem to work! www.biorxiv.org/content/10.1... Code: github.com/paulrobustel...

submitted 126 days ago • 2 comments

Structure of a zoonotic H5N1 hemagglutinin reveals a receptor-binding site occupied by an auto-glycan pubmed.ncbi.nlm.nih.gov/39884273/ #cryoEM

submitted 126 days ago • 0 comments

Researchers used AlphaFold alone (no wet-lab experiments; no cryo-EM etc.) to build a structural model and predict how a huge complex in cells, made of 17 proteins, spans across the bacterial cell envelope to transduce force for motility. Can anyone tell me if this is a first?

submitted 127 days ago • 1 comment

I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...

submitted 135 days ago • 11 comments

It was a pleasure to host Professor Wolfgang Baumeister and share the latest results on developing an #ultrafast #laser #phaseplate for #cryoEM and #cryoET from @fitzpatricklab.bsky.social @zuckermanbrain.bsky.social

submitted 140 days ago • 0 comments

Kexin Zhang et al.: A new statistical metric for robust target detection in cryo-EM using 2D template matching #3DReconstructionAndImageProcessing #SingleParticleCryoEM #Imaging @umassboston...#IUCr https://journals.iucr.org/paper?S2052252524011771

submitted 140 days ago • 0 comments

Paper of interest for the #CryoET folks. www.nature.com/articles/s41...

submitted 147 days ago • 1 comment

For all #cryoEM practitioners in #NYC, @fitzpatricklab.bsky.social is excited to host Prof. Wolfgang Baumeister next Thursday with attendance open to all and no registration necessary. Hope to see you there!

submitted 148 days ago • 1 comment

Excited to present our lab's new work on GPCR antagonism: Contrary to popular belief, we propose that inverse agonists can bind to and act via GPCR:G protein complexes. We show this at KOR, through cellular assays, radioligand binding and cryoEM studies. A thread. 💊 🧪 www.nature.com/articles/s41...

submitted 150 days ago • 2 comments

This was a great collaboration and culmination of a huge amount of work including our first attempt to join #teamtomo. @ruizhangmt.bsky.social expertly summarizes the highlights below:

submitted 155 days ago • 0 comments

Merry Christmas and our best wishes for a peaceful 2025. Say goodbye to the noise with CryoSamba, our self-supervised deep denoising algorithm for CryoET now published in JSB.

submitted 160 days ago • 1 comment

rdcu.be/dY5rl The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.

submitted 162 days ago • 1 comment

Bioart, courtesy of the NIH. All images are in the public domain and available for download as high-quality SVGs. bioart.niaid.nih.gov

submitted 172 days ago • 14 comments

Hello, old and new friends in Bluesky. Using cryo-FIB-ET, we revealed a 9Å structure, identifying novel layers and conserved interfaces that may serve as antiviral targets. #Ebola #Virology #StructuralBiology The full manuscript is available here authors.elsevier.com/sd/article/S...

submitted 165 days ago • 0 comments

An opioid with a literal, light-sensitive 'on/off switch' to avoid fatal side effects? Look no further - Azomorphine-3 (AM3)! The most exciting study I've been part of, thus far. With the labs of Seva Katritch, Dirk Trauner @dirktrauner.bsky.social and Josh Levitz. www.biorxiv.org/content/10.1...

submitted 168 days ago • 4 comments

The @structurabio.bsky.social team have been preparing Amazing workshops and tutorials on all things related to cryo-EM image analysis, demonstrating deep talent in communication in addition to software design. If you're interested in learning about cryo-EM do yourself a favour and have a look!

submitted 175 days ago • 2 comments

This new resource is AMAZING. You can easily search (re-search) your proteomics data in the context of human genomics (you know, that whole thing where we don't all have the exact same genome?) Read the paper, but instructions in the blog post - proteomicsnews.blogspot.com/2024/12/proh...

submitted 178 days ago • 6 comments

Looking for a primer on why the contrast transfer function matters in #cryoEM? Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...

submitted 179 days ago • 0 comments

Check out our updated database of 189 PhD fellowships and funding opportunities. For each fellowship, we provide a description, $ amount, deadline, link to funder and eligibility criteria (such as citizenship). Good luck! Download freely here: research.jhu.edu/rdt/funding-...

submitted 199 days ago • 1 comment

If you are interested or working in #structuralbiology, as you see any protein (yes, any), you should be thinking: is it glycosylated? The answer is likely to be yes! You can predict N-glycosylation sites and rebuild all types of glycans with ReGlyco glycoshape.org 😌 Check out the example ⬇️ 1/2

submitted 200 days ago • 4 comments

For people interested in chaperones, protein quality control, proteostasis and cellular homeostasis, here is the link of the webinars we (P. Goloubinoff, A. Barducci, N. Nillegoda and I) hosted during the COVID pandemics. A lot of material! tinyurl.com/27y5wpzy

submitted 201 days ago • 2 comments

Download our database of 492 (!) funding opportunities for EARLY-CAREER FACULTY AND RESEARCHERS. For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship). Download freely here: research.jhu.edu/rdt/funding-...

submitted 201 days ago • 12 comments

🔬 Are you eager to dive into the world of light microscopy & learn from top-notch microscopists about its essential techniques for life science applications? If that sounds exciting, don’t miss out on this free, in-depth iBiology series! 👉 www.ibiology.org/online-biolo...

submitted 202 days ago • 17 comments

Now out in Nature Comms! Sarah Bickers (Kanelis lab and Rubinstein lab) determines the structure of the dimeric form of the ABC protein Ycf1p. www.nature.com/articles/s41...

submitted 202 days ago • 0 comments

Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/ G. Napolitano,A. Esposito, and A. Ballabio.

submitted 202 days ago • 8 comments

Our latest #preprint on the development of an #ultrafast #laser #PhasePlate for #cryoET: biorxiv.org/content/10.1... #femtosecond #laser #optics #UEM #SEM #cryoEM experiments made possible by generous support from @cziscience #VisualProteomics #ImagingTheFuture

submitted 437 days ago • 1 comment