jnpruneda.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

If you always wanted to know the kinetics of how the Polycomb enzymes modify chromatin, we are thrilled to share our work!!! 🎉📄🧬🔬 Thank you, @beatfierz.bsky.social for all the guidance as well as the @lcbm-epfl.bsky.social group members for all the support! doi.org/10.1126/scia...

submitted 1 day ago • 1 comment

Out in @pnas.org today Showcasing the power of NMR! NMR and enzyme kinetics show USP7 enzyme transiently sampling its low-populated active state, and its hyperactive disease variant enhancing these dynamics www.pnas.org/doi/10.1073/... Stellar work by students Gabrielle Valles and Emilie Korchak

submitted 2 days ago • 0 comments

Very happy to share two back-to-back preprints of my postdoc work on bacterial NEL E3 ligases from my time in @katrinrittinger.bsky.social lab at @crick.ac.uk in collaboration with the ChemBio team at GSK (1/4)

submitted 2 days ago • 1 comment

Have you ever wondered how repeated vaccination shapes our immune responses, and whether timing between doses matters? Check out our latest work! @ohsunews.bsky.social @ohsummi.bsky.social www.nature.com/articles/s43...

submitted 4 days ago • 0 comments

Another awesome collaboration with @jnpruneda.bsky.social and team providing deeper insight into the writers of MARUbylation. Check out thread 👇!

submitted 6 days ago • 0 comments

Sharing the next installment of our fun collaboration with @michaelnadbio.bsky.social at the interface of #ubiquitin and #ADP-ribosylation! Building upon our identification of cellular MARUbylation, we now identify reader/writer E3 ligases that extend K11 polyUb! 🧵 www.biorxiv.org/content/10.1...

submitted 6 days ago • 4 comments

How can a Salmonella effector change the specificity of a host kinase? SteE mediates kinase reprogramming via mimicry of conserved eukaryotic-like motifs- doi.org/10.1038/s443... Great collaboration with @katrinrittinger.bsky.social + big thanks to all authors & congrats to lead PDRA Ioanna Panagi!

submitted 10 days ago • 0 comments

Great to see this out. A story that spanned continents, cities and budget codes… www.nature.com/articles/s41... Big thanks to all the teams involved! @clausenlab.bsky.social @e3chembio.bsky.social

submitted 10 days ago • 3 comments

Excited that our work revealing the structure and function of the monster stress response silencing factor, the E3 ligase SIFI, is now out! Congratulations to the most amazing team of Zhi Yang, Diane Haakonsen, Michael Heider and Sam Witus! www.nature.com/articles/s41...

submitted 16 days ago • 1 comment

Super happy to share that our work on the specific inhibition of USP30 - a clinical stage Parkinson's drug target - is now online at NSMB www.nature.com/articles/s41... Have a read if you fancy chimeric protein engineering or a framework for DUB ligandability. Huge congratulations to Nafizul & team!

submitted 18 days ago • 3 comments

Want to learn more about chromatin and its dynamics? Read this review by Charlotte and Jolijn in our lab, aimed at giving an overview on this and the ways this is dealt with during transcription, replication and damage repair. Thanks to Biochemistry for inviting us! doi.org/10.1021/acs....

submitted 19 days ago • 2 comments

Congratulations to the groups of Eric Barklis and @tafesselab.bsky.social for reporting an analysis of factors that regulate HIV-1 fusion in reverse pmc.ncbi.nlm.nih.gov/articles/PMC...

submitted 23 days ago • 0 comments

How do cells keep their cilia “clean” and functional? Our new study uncovers a conserved mechanism for retrieving polyubiquitinated proteins from #cilia – a process essential for cellular signaling and health. #cellbiology #ciliopathy #ubiquitin #IFT 🧵👇 1/n

submitted 24 days ago • 2 comments

I would like to share our latest work: After years of optimisation, we have developed a flow cytometry-based method to isolate pathogen-containing phagosomes. Paired with sensitive proteomics, this provides exciting new insights into host-pathogen interactions! www.biorxiv.org/content/10.1...

submitted 24 days ago • 0 comments

Pfs48/45 nanobodies block transmission 🦟 Pfs48/45 Nb-Fc reduce oocyst numbers 💎 Structure of transmission-blocking Nb with Pfs48/45 👐🏽 Bispecific Nb-Fc targeting both Pfs230 and Pfs48/45 🧑🏻‍🔬 PhD student Frankie Lyons 1st Author 🥳 Please repost and happy reading! www.biorxiv.org/content/10.1...

submitted 27 days ago • 1 comment

Thanks @kfeijszaja.bsky.social and team for the excellent Spotlight on our collaborative work with @jnpruneda.bsky.social: Modifying the modifiers: ubiquitination of ADP-ribosylation in human cells: Trends in Biochemical Sciences www.cell.com/trends/bioch...

submitted 29 days ago • 2 comments

New role for macrophages in Shigella infection control @cgmargarida.bsky.social @lshtm.bsky.social @cp-cellreports.bsky.social www.cell.com/cell-reports...

submitted 29 days ago • 0 comments

Happy to see our review out in ChemBioChem @chemistryeurope.bsky.social in which we describe the tools and strategies for deciphering linkage type-specific #ubiquitin signalling: doi.org/10.1002/cbic.... Thanks to Julian for spearheading this, and to Camilla and Dominik for important contributions.

submitted 31 days ago • 1 comment

Another insightful piece recently out @natsmb.nature.com delineating how Shigella utilises its effector IpaH1.4 to counteract host RNF213-induced LPS ubiquitylation. Very interesting work by the lab of @felixrandow.bsky.social. If you want to, have a go at it: www.nature.com/articles/s41...

submitted 38 days ago • 0 comments

Finally for today, a collaborative effort led by the lab of @zeqiraj.bsky.social discovers molecular glues for BRISC, a deubiquitylating enzyme regulating inflammatory signalling. A delightful read here: www.nature.com/articles/s41... and the authors original thread here: bsky.app/profile/zeqi...

submitted 38 days ago • 0 comments

We @natsmb.nature.com recently published an insightful manuscript on ubiquitin-independent proteasomal degradation via the NUB1-FAT10 axis by the lab of Andreas Martin. A very interesting read found here: www.nature.com/articles/s41...

submitted 38 days ago • 0 comments

The Coers lab is now on bsky.

submitted 39 days ago • 1 comment

Come and work with me! The Nature Micro team is expanding and we're looking for someone to champion microbial ecology, plant micro & related areas for the journal Knowledge of microbial ecology/plant micro is desirable but we're open to applications from all microbiologists Link below 👇

submitted 42 days ago • 1 comment

Happy to share my Ph.D work!! Our latest paper shows VCP/p97 uses its tweezer-like action to tear invading bacteria apart — a powerful cellular defense! 🦠 @natmicrobiol.nature.com @BsbeIitb @iitbombay @CSIR This was years of hard work! Thanks all🙌 #microbiology #cellautonomous #immunity #ubiquitin

submitted 41 days ago • 0 comments

After a great review process with @naturecomms.bsky.social, I'm so happy to share our work identifying inactive RING E3 #ubiquitin #pseudoligases in the TRIM protein family!! rdcu.be/ehlGW 🥳 🍾 🙌 @katmcphie.bsky.social @diegoesposito.bsky.social @katrinrittinger.bsky.social @crick.ac.uk

submitted 42 days ago • 3 comments

Excited to share our latest work, now out in @lsajournal.org. we show that the E3 ligase HOIL-1 can ubiquitinate many different saccharides and describe a chemoenzymatic platform to generate ubiquitinated sugars as tools for studying non-proteinaceous ubiquitination.

submitted 43 days ago • 1 comment

RNF213, a host E3 ligase, ubiquitylates LPS to mark cytosol-invading bacteria for autophagy. But how do cytosol-adapted bacteria escape? We found that Shigella's E3 ligase IpaH1.4 blocks LPS ubiquitylation by degrading RNF213 via the proteasome. www.nature.com/articles/s41...

submitted 44 days ago • 2 comments

Great news: NIH postbac program is recruiting again! If your grad school plans were affected by program cutbacks or admissions freezes this year I highly encourage you to apply, this could be a perfect opportunity. Please repost. www.training.nih.gov/research-tra...

submitted 44 days ago • 5 comments

New Preprint: We have solved the structure of the proteasome in complex with two different ubiquitin chains: www.biorxiv.org/content/10.1... This work is spearheaded by PhD Student Sascha Amann Let me walk you through it 🧵 1/9

submitted 44 days ago • 2 comments