reptilerhett.bsky.social
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Field Applications Bioinformatic Support (FABS) Scientist @pacbio.bsky.social
PostDoc: Tasmanian Devils @wsupullman.bsky.social & @usouthflorida.bsky.social
PhD: š venom evolution @SnakeLabClemson
22 posts
94 followers
135 following
Prolific Poster
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Typically I would recommend hifiasm for assembly. Itās fast and produces the highest quality assemblies.
Hope this helps!
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Hi! PacBio bioinformatics support scientist here specializing on genome assembly.
QC reports are mainly generated on-instrument or in SMRT Link. Whoever you did your sequencing with, should be able to provide you with a QC report. There is no trimming or external QC needed.
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www.pacb.com/technology/p...
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PacBio has a product called PureTarget for Cas9 based pull-down. The kit is specific to 20-ish repeat expansion genes in the human genome, but could be customized.
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Jealous.
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You get a T2T assembly! You get a T2T assembly! Everybody gets a T2T assembly!
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American academicsā¦.