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srouhanifard.bsky.social
Asst. Prof of bioengineering @Northeastern University | RNA modifications | RNA localization | click chemistry | single-molecule studies | www.rouhanifardlab.com
8 posts 58 followers 84 following
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1/6 🧬New paper at @cp-cellsystems.bsky.social led by @sashafanari.bsky.social! Does pseudouridine (Ψ) dynamically respond to cell state? We used nanopore DRS + Mod-p ID to map Ψ in neuron-like cells under “normal” and “perturbed” conditions (healthy differentiation and unhealthy Pb2+ poisoning).

submitted 64 days ago • 1 comment

Sitting in a journal club-style class right now and after the main presenters present, there are "roles" from others in the group, like the "archeologist" who looks into the citation trail, the "private investigator" who tries to dig into the history, and the "industry practitioner", etc. Cool idea!

submitted 67 days ago • 4 comments

🧪🧬👩‍🔬🖥️ Do immortalized cells have a different RNA modification profile compared to primary cells? 🤔 @sashafanari.bsky.social @wanunupore.bsky.social @srouhanifard.bsky.social assess the most abundant RNA modification, pseudouridine, in primary and immortalized human T cells 👇 doi.org/10.1101/2025...

submitted 75 days ago • 0 comments

Direct RNA sequencing of primary human T cells reveals the impact of immortalization on mRNA pseudouridine modifications. https://www.biorxiv.org/content/10.1101/2025.03.02.641090v1

submitted 75 days ago • 0 comments

Office hours are starting

submitted 92 days ago • 0 comments

Online Now: Identification of molecular determinants of gene-specific bursting patterns by high-throughput imaging screens

submitted 94 days ago • 0 comments

Single-molecule live-cell RNA imaging with CRISPR–Csm - @doudna-lab.bsky.social @qb3-berkeley.bsky.social @innovativegenomics.bsky.social go.nature.com/3EJPKsM

submitted 95 days ago • 2 comments

Cool paper using LLM to discover a protein sequence code for subcellular localization 👏 www.science.org/doi/10.1126/...

submitted 105 days ago • 6 comments

Gene editing technology began by people studying salt marshes. Ozempic began by folks studying the venom of Gila Monsters. Support for basic science has empowered us to understand our world. Tethering it to applications health has transformed and saved countless lives.

submitted 105 days ago • 50 comments

Check it out! Another novel RNA modification - ADP-ribosylation - that was previously only known on proteins. A cousin to #glycoRNA 👏 www.nature.com/articles/s41...

submitted 107 days ago • 3 comments

"Imagine a DAPI-like stain, but for the extracellular matrix." That's basically how this work was pitched to me by Kayvon and Antonio a year or so ago. Now the final product really delivers. Read about their versatile label for ECM in living tissues here: www.nature.com/articles/s41...

submitted 107 days ago • 9 comments

Liu, ... Rouhanifard have optimized nuclear FISH to the point where it's now possible to sort cells based on transcript production. They sort cells into bins and measure chromatin state at the locus whose tx was sorted on. www.biorxiv.org/content/10.1...

submitted 136 days ago • 2 comments