srouhanifard.bsky.social
Asst. Prof of bioengineering @Northeastern University | RNA modifications | RNA localization | click chemistry | single-molecule studies | www.rouhanifardlab.com
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6/6 🙌 Future work will determine whether static sites are critical for cellular function while plastic sites fine-tune gene expression in response to environmental stressors.
Huge thanks to the team for all their hard work! @wanunupore.bsky.social @genometdcc.bsky.social
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5/6 ⚙️ Enzyme insights: TRUB1 and PUS7 levels shifted with differentiation, and KD experiments reveal unexpected coregulation—knocking down one lowers Ψ at the other’s targets. This hints at a coordinated network fine-tuning mRNA Ψ for neuronal homeostasis and stress resilience.
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4/6 📊 Plasticity vs stability: ~30% of Ψ sites changed occupancy across conditions (“plastic”), while the rest remained constant (“static”). Among the plastic sites was a key Ψ site in YTHDF1—an m6A reader involved in translation, which changed in response to cellular and environmental cues.
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3/6 📊 Key finding #1: Pb²⁺ treated cells had more Ψ sites transcriptome-wide but lower relative occupancy per site vs untreated/differentiated cells—suggesting a broad but shallow protective pseudouridylation response to stress.
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2/6 🧠Link here: authors.elsevier.com/a/1koBC8YyDf...
Method: SH SY5Y cells were untreated, differentiated with retinoic acid, or exposed to Pb²⁺. Nanopore DRS determined site-specific, relative Ψ “occupancy”; orthogonal knockdowns (TRUB1, PUS7) and biochemical assays validated sites.
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I sat on a study section that met this past Friday (2/14)