tonnigrubeandersen.bsky.social
MPG group leader and AvH fellow with interests in development, physiology and CLSM. I need to know how 🌱 communicate with environment. privately: huge cat-fan
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Thank you Jen! Means a lot coming from you! :-)
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Wound be fun to figure out where on the root this occurs ;-)
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Thanks Peter!
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Thanks AP! ☺️
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Thank you Tatsuya ☺️ likewise with your talk!
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Thank you Jim!
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Thank you for reading this far! If you are interested in more details, feel free to contact me or @leoniekraska.bsky.social . Warning: We can both talk about this for hours, so make sure you have enough time if you do so! :-)
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We made this summary model that also integrates MYB68 into periderm and middle cortex formation, which is an essential part of our sister manuscript headed by Lauras group, so a continuation is soon to come. (you can check the preprint here: www.biorxiv.org/content/10.1... )
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There is a lot more super cool findings in this paper, including specific localization of MYB68 to phloem-pole associated endodermis (PPE), which may explain its role as a passage cell inhibitor, although we don't know the mechanism... yet..
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Since Leonie is unstoppable, she decided to look deeper into this using Fluorescent in-situ Hybridization techniques to visualize mRNA. She found direct evidence that several K-transporters including HAK5 is expressed in passage cells:
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These included some surprises that you can read in the paper, but also genes associated with gibberelin and, excitingly, also cation transport!!
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We realized that this could be used to identify what these elusive cells do, and we embarked on a transcriptional adventure to figure out which genes correlate with the passage cell behavior under standard and under cytokinin-treatment in the myb68 KO roots. This way, we got ~300 new markers!
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She thereby realized MYB68 may be related to passage cell formation and not directly suberization - a finding that was confirmed by measurement of the passage cell marker PHO1;H3, which was strongly increased in the endodermis of myb68 KO roots
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What was super cool is the the XPE hosts so-called "passage cells" that are sensitive to low amounts of cytokinin, which represses their formation. Leonie found that the pattern of myb68 KO root suberization could be reversed when germinated on the artificial cytokinin BAP. a very exciting hint!
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Leonie went all-in and described this change in suberin patterning - down to single cells along the entire root! - it took about 2 months of imaging to get this data and describe the pattern with such detail, but turns out that the effect is spatially restricted to certain cell files along the root
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This turned out to be a strong decrease in suberization of specifically the xylem pole-associated endodermis (XPE). A very exciting pattern, which may explain why suberin is a dynamic process that is always hard to describe correctly.
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Leonie wanted to find new regulators involved in a process called "suberization" - where the endodermal cells in the root close to minimize transport of molecules. She discovered that MYB68 - which is related to the Casparian strip regulator, had a peculiar pattern of suberin deposition when KOed: