The preprint of my main thesis work is live! #condensin #chromatin #generegulation #celegans 🪱 www.biorxiv.org/content/10.1... - ThreadSky

springcyclone.bsky.social • 20 days ago

The preprint of my main thesis work is live! #condensin #chromatin #generegulation #celegans 🪱

https://www.biorxiv.org/content/10.1101/2025.02.05.636654v1

Comments

needhibhalla.bsky.social•19 days ago

Congratulations to you and all the authors! 🎊🎉🥳

springcyclone.bsky.social•19 days ago

Thank you! 🥺

springcyclone.bsky.social•20 days ago

Here, we started with that premise that worms have an extra SMC protein, DPY-27, in the dosage compensation specific condensin I-DC

Q: Can DPY-27 hydrolyze ATP like other SMC proteins and is this function required for dosage compensation?

springcyclone.bsky.social•20 days ago

This question came out of a debate – is condensin I-DC an active condensin OR is it just a scaffold for the rest of the dosage compensation complex?

So we said, let's kill the ATPase and see what happens. Luckily for us, DPY-27 is really similar to other SMC proteins

springcyclone.bsky.social•20 days ago

So Dillon, our tech, made the mutant worm 🪱

We actually ended up with 2 worms, the ATPase dead mutant (EQ) and an insert-deletion (indel) which phenocopied other condensin null mutants. We decided to use the indel as comparison for the ATPase dead mutant

springcyclone.bsky.social•20 days ago

But then I threw a wrench into the whole story by saying "let's do the in vitro biochemistry" 🤡 our lab is a not a biochemistry lab so I had to learn everything from the ground up 🙃

(there were some days of lying on the ground and contemplating existence)

springcyclone.bsky.social•20 days ago

The in vitro biochemistry demonstrates that DPY-27 can hydrolyze ATP and that the EQ mutation abolishes this function

Our mutant worms demonstrate that losing this ATPase function is equally severe to losing the functional protein (EQ vs indel worms).

springcyclone.bsky.social•20 days ago

We also found that loss of this function can prevent condensin I-DC from strongly binding DNA but it is random, leaving 2 populations – strongly DNA bound and weakly DNA bound, contributing to the lack of dosage compensation and maternal effect lethality in offspring

Comments

Posting Rules

Reply