Exciting study by Amanda Andersson Rolf et al: In vitro expansion of a human fetal #pancreas #stemcell that generates all three pancreatic cell lineages”. www.cell.com/cell/fulltex... New #organoid protocol allows the seemingly indefinite expansion of human fetal pancreatic Lgr5+ stem cell 1/ - ThreadSky

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hansclevers.bsky.social • 84 days ago

Exciting study by Amanda Andersson Rolf et al: In vitro expansion of a human fetal #pancreas #stemcell that generates all three pancreatic cell lineages”. https://www.cell.com/cell/fulltext/S0092-8674(24)01254-6
New #organoid protocol allows the seemingly indefinite expansion of human fetal pancreatic Lgr5+ stem cell 1/

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hansclevers.bsky.social•84 days ago

The 3 epithelial compartments of the pancreas are acini, ducts and islets of Langerhans. The first two form the exocrine pancreas, producing digestive enzymes to be transported to the duodenum. The endocrine islets secrete hormones (e.g. insulin, glucagon) into the circulation 2/

hansclevers.bsky.social•84 days ago

Mouse studies indicate that these three compartments derive from a transient, common pancreatic progenitor. Amanda et al derive 18 human fetal pancreas organoid (hfPO) lines from gestational week 8-17 samples. Four lines generate acinar-, ductal- and endocrine lineage cells 3/

hansclevers.bsky.social•84 days ago

The four lines expand exponentially for >2 years under optimized culture conditions. Single cell RNA seq identifies rare LGR5+ cells in fetal pancreas and in hfPOs as the root of the developmental hierarchy. These LGR5+ cells share multiple markers with adult gut stem cells 4/

hansclevers.bsky.social•84 days ago

Clonal organoids grown from a single LGR5+ cell recapitulate this tri-potency in vitro. Our study describes a human fetal tri-potent stem cell, capable of long-term expansion in vitro and of generating all three pancreatic cell lineages 5/

hansclevers.bsky.social•84 days ago

3D Reconstruction of human fetal pancreatic organoid with an endogenous (knock-in) reporter for the endocrine cell marker Chromogranin-A (green), while stained for the acinar cell marker CarboxyPeptidase A1/2 (magenta) and nuclei, DAPI (blue)

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