As many labs are starting to implement these stem cell models, it is becoming increasingly important to define key experimental variables and address challenges in conducting, reporting, and evaluating in vitro and in vivo studies. Open article here: www.nature.com/articles/s41... - ThreadSky

sergiuppasca.bsky.social • 75 days ago

As many labs are starting to implement these stem cell models, it is becoming increasingly important to define key experimental variables and address challenges in conducting, reporting, and evaluating in vitro and in vivo studies.

Open article here:
https://www.nature.com/articles/s41586-024-08487-6

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sergiuppasca.bsky.social•75 days ago

Following an initial effort to reach consensus on the nomenclature, we gathered a group of researchers and, for over a year, we worked to outline a framework that can apply to neural #organoids and #assembloids and their xeno-transplantation.

A summary of our recommendation can be seen here:

sergiuppasca.bsky.social•75 days ago

First, we emphasize how critical it is to establish the quality of hiPS cells. We also point out issues to consider including accumulating mutations, X chromosome inactivation, use of isogenic versus patient lines and the need for creating a collection of broadly consented, well-characterized lines

sergiuppasca.bsky.social•75 days ago

Second, we outline experimental variables related to neural differentiation including implementing quality control steps at key stages to enhance reliability (and save resources) and make recommendations for characterization and validation of new differentiation protocols. see Box here:

sergiuppasca.bsky.social•75 days ago

Third, we discuss the probing & manipulation of neural cells, including with transcriptomics, calcium imaging and electrophysiology

We also point out that to substantiate claims about genetic background in disease models, a large number of independent lines is required as well as orthogonal assays

sergiuppasca.bsky.social•75 days ago

Fourth, we consider aspects of experimental design for transplantation of human neural cells into host organisms to mitigate some limitations of in vitro systems and mention that ethical, moral, societal and legal concerns are the focus of ongoing discussions (more on that in a separate article)

sergiuppasca.bsky.social•75 days ago

Fifth, we recommend rigorous benchmarking, robust statistical and experimental design, and taking into considerations the limitations of these models when interpreting data.
Depositing raw data in public databases, describing experimental details in papers, and using standardized terms is critical.

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