Tracking #microtubule tips in plant cells on a @zeiss-microscopy.bsky.social LSM880 #confocal #microscope 🌱🔬 #aqlm2025 #microscopymonday #microscopy #plantscience #botany #cytoskeleton #cellbio - ThreadSky

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nat-prunet.bsky.social • 52 days ago

Tracking #microtubule tips in plant cells on a @zeiss-microscopy.bsky.social LSM880 #confocal #microscope 🌱🔬

#aqlm2025 #microscopymonday #microscopy #plantscience #botany #cytoskeleton #cellbio

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fabienmiart.bsky.social•51 days ago

Thanks for sharing Nat! Microtubules under a microscope are so beautiful! Is it spinning disk?! TIRF?

nat-prunet.bsky.social•43 days ago

also TIRF wouldn't work as the cell wall is thicker than the evanescent wave

fabienmiart.bsky.social•42 days ago

Sorry for my late response Nat! It worked well for me with microtubule and cellulose synthase imaging. But you're right, finding the exact critical angle can be a bit tricky!

fabienmiart.bsky.social•42 days ago

I’d actually recommend using VAEM (Variable Angle Epifluorescence Microscopy), where the mirror angle can be adjusted and it’s still very effective at reducing background noise!

nat-prunet.bsky.social•51 days ago

Point-scanning confocal. It would look so much better on a spinning disk! But the goal is for students to see to pros and cons of different techniques

nat-prunet.bsky.social•52 days ago

#microtubule tip tracks color-coded for time

Imaged on a @zeiss-microscopy.bsky.social
LSM880 #confocal #microscope 🌱🔬

#aqlm2025 #microscopymonday #microscopy #plantscience #botany #cytoskeleton #cellbio

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