In 2019, Hoffmann and Judkewitz showed that a diffraction grating could be used to redirect the light into the tertiary objective, but this led to a distorted point spread function and low photon efficiency. doi.org/10.1364/OPTI... - ThreadSky

jamesdmanton.bsky.social • 15 days ago

In 2019, Hoffmann and Judkewitz showed that a diffraction grating could be used to redirect the light into the tertiary objective, but this led to a distorted point spread function and low photon efficiency.

https://doi.org/10.1364/OPTICA.6.001166

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jamesdmanton.bsky.social•15 days ago

More recent work, e.g. by Shao et al. and Daetwyler et al., have extended this concept, but the relatively low photon efficiency and difficult alignment procedure remain.

https://doi.org/10.1364/OPTICA.471101
https://doi.org/10.1364/OPTICA.502243

jamesdmanton.bsky.social•15 days ago

Hoffmann et al. have also shown how an image transfer fibre plate can be used to achieve a similar goal without the photon losses of diffractive elements, but this requires a costly custom-made optical element.

https://doi.org/10.1038/s41467-023-43741-x

jamesdmanton.bsky.social•15 days ago

In our work, we just put the camera directly into the remote refocus volume to detect the oblique plane directly, without the need for a tertiary microscope system!

jamesdmanton.bsky.social•15 days ago

As a proof-of-concept, Jacob Lamb built a direct-view mesoscopic OPM using a €700 Ximea MU196MR-ON camera with 1.4 μm pixels. We could then image expanded cerebral organoids, created by @miguelcmestre.bsky.social, with a 2 μm × 2 μm × 22 μm resolution over a 5.3 mm × 2.88 mm field of view.

jamesdmanton.bsky.social•15 days ago

We hope our concept will significantly democratise access to high-throughput imaging of tissue samples at sub-cellular resolution and, with continued developments in camera technologies, might become a viable technique for high-resolution single-cell OPM imaging without the need for a 3rd objective.

retof.bsky.social•15 days ago

Ahem…

Acknowledgement: JRL and JDM thank Taylors of Harrogate for producing the calming beverage Yorkshire Tea, consumed after sensor cover glass removal.

retof.bsky.social•15 days ago

Congrats! Happy to see this out on BioRxiv.

How hard was it to modify the camera?

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