Our technique for studying strain-level IgA binding in the microbiome is out now at @naturemicrobiol.bsky.social! Fascinated by IgA since Sean Spencer told me about it, here we make some fundamental discoveries about strain-level IgA dynamics in the healthy human gut (1/5) doi.org/10.1038/s415... - ThreadSky

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mattolm.bsky.social • 144 days ago

Our technique for studying strain-level IgA binding in the microbiome is out now at @naturemicrobiol.bsky.social! Fascinated by IgA since Sean Spencer told me about it, here we make some fundamental discoveries about strain-level IgA dynamics in the healthy human gut (1/5) https://doi.org/10.1038/s41564-024-01887-4

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ppenev.bsky.social•142 days ago

Very interesting paper! Do you happen to have an open access version of the full paper?

mattolm.bsky.social•144 days ago

IgA is the main antibody secreted by mucosal surfaces and it binds ~15% of human gut microbes. Binding can be both beneficial or detrimental to microbes, and there's lots we don't know about who IgA binds and how it sculpts the microbiome. We developed MIg-Seq to answer some of these questions (2/5)

mattolm.bsky.social•144 days ago

We found discrete IgA binding levels, consistency in microbial IgA binding across people (columns), & intriguing trends in IgA binding across species (rows). We spent hours staring at this first ever comprehensive genome-resolved view of IgA binding; so much insight and so much to learn! (3/5)

mattolm.bsky.social•144 days ago

We also found that there are specific genes linked to binding (indicating that microbes in the mucosa are typically targeted) (Fig. 3), that IgA-bound microbes have lower replication rates (Fig. 4), and that IgA binding is highly integrated with other components of the immune system (Fig. 5) (4/5)

mattolm.bsky.social•144 days ago

In my new lab at CU Boulder we are exploring how IgA binding shifts in infancy and states of disease, and the extent to which IgA actively shapes the human microbiota. Wet-lab protocol at https://doi.org/10.17504/protocols.io.5jyl8pk6dg2w/v1; bioinformatics code at https://github.com/MrOlm/MIGSeq_code. Thanks for reading! (5/5)

ngsorcerer.bsky.social•144 days ago

This is amazing! Could you provide some more detail on the IgA+ probability score? How were the thresholds chosen, and does the score roughly reflect a specific quantity/density/signal of bound IgAs?

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