Really excited to share our new paper!! We describe a detailed protocol for setting up dissociated mouse myelinating Schwann cell dorsal root ganglion neuron cocultures in microfluidic chambers, and use this model to investigate axon degeneration. journals.biologists.com/jcs/article/... - ThreadSky

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claramutschler.bsky.social • 519 days ago

Really excited to share our new paper!! We describe a detailed protocol for setting up dissociated mouse myelinating Schwann cell dorsal root ganglion neuron cocultures in microfluidic chambers, and use this model to investigate axon degeneration. https://journals.biologists.com/jcs/article/136/18/jcs261557/329062/Schwann-cells-are-axo-protective-after-injury

Comments

operationoxygen.bsky.social•511 days ago

Wonderful work! We need to read this.

claramutschler.bsky.social•509 days ago

Thank you!!

claramutschler.bsky.social•519 days ago

In our model, we achieve beautiful myelination, and replicate key parts of the Schwann cell injury response, such as upregulation of the transcription factor JUN, demyelination of degenerating axons, and ingestion of axonal fragments.

claramutschler.bsky.social•519 days ago

We also show that Schwann cells are axo-protective after injury in these cocultures, regardless of whether they are myelinating or not.

claramutschler.bsky.social•519 days ago

If you want to read a short summary of the work, check out this research highlight
https://journals.biologists.com/jcs/article/136/18/e136_e1801/329426/A-new-coculture-system-for-mouse-myelinating

claramutschler.bsky.social•519 days ago

And if you want to know a little bit more about me – hi, it’s me!

https://journals.biologists.com/jcs/article/136/18/jcs261615/329060/First-person-Clara-Mutschler

claramutschler.bsky.social•519 days ago

Also, massive thanks to all my co-authors – couldn’t have done it without you! @colemanlab.bsky.social (...still waiting for the rest of you to join me over here👀)

jdpereira.bsky.social•519 days ago

That is gorgeous!!

claramutschler.bsky.social•518 days ago

Thank you!! I pretend I do this for the science but really it’s all for the pretty pics😍

jdpereira.bsky.social•518 days ago

Same!!

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