Here is a sneak peak of the new sequencing technology I have been working on together with many others. Much more to come in 2025! www.illumina.com/science/geno... - ThreadSky

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shunhuahan.bsky.social • 102 days ago

Here is a sneak peak of the new sequencing technology I have been working on together with many others. Much more to come in 2025!

https://www.illumina.com/science/genomics-research/articles/constellation-mapped-read-technology.html

Comments

gringene.org•102 days ago

How do you guarantee that template DNA is consistently fragmented into lengths that work well for bridge amplification and reliable spot detection?

Is the flow cell loaded with a huge excess of transposase?

shunhuahan.bsky.social•102 days ago

Full details on the prep and computational process will be shared later, stay tuned :)

msikic.bsky.social•101 days ago

Interesting. Is there a distribution or read lengths, and is it possible to use them beyond mapping strategies, i.e. de novo assembly?

shunhuahan.bsky.social•101 days ago

The template size at 99th percentile from HMW extraction is typically 100-300kb with some greater than 300kb. Full details will be publicly shared later.

Yes, one could design a de novo assembly process that takes advantage of the templates. The algorithm side of this technology is super fun :)

msikic.bsky.social•101 days ago

Thanks. Sorry I didn't get about size. 1 percent of reads are longer than 100 k or 99%. How much HMW DNA is required? I see, there is a need to develop algorithms from the scratch. Yes that might be interesting. Actually, for de novo assembly only overlap needs to be re-done

shunhuahan.bsky.social•101 days ago

1% for the first question. Details on extraction will be shared later! :)

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