chicalab.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

How do mutations far from an enzyme's active site influence catalysis? 🤔 Part 2: In collaboration with @fraserlab.bsky.social and @silviaosuna.bsky.social, we investigated this question using de novo Kemp eliminases, revealing effects of distal mutations on the catalytic cycle. 🧵 (1/6)

submitted 3 days ago • 1 comment

Whereas beneficial active site mutations to enzymes often improve the chemical transformation itself by preorganizing the active site, mutations to second-shell residues instead tune steps like product release by modifying the broader conformational ensemble www.biorxiv.org/content/10.1...

submitted 3 days ago • 1 comment

How do distal mutations contribute to enzyme catalysis? Our new study, in collaboration with @thompson-lab.bsky.social, Marc Garcia-Borràs, and @ferranfeixas.bsky.social, reveals how they optimize structural dynamics & local electric fields to drive the catalytic cycle. 🧵👇

submitted 4 days ago • 1 comment

Join us this summer for 3.5 days for science! The Protein Society's 39th Annual Symposium takes place June 26 - 29, in beautiful San Francisco. #proteinscience #annualsymposium #proteinsociety mailchi.mp/proteinsocie...

submitted 26 days ago • 0 comments

Thrilled to take a break from doom and gloom to share our latest work collaborating with the DeGrado lab! We took a de novo designed protein, screened it for ligand binding using X-rays, and used the hits to evolve two wildly different functions: fluorescent turn-on and Kemp elimination catalysis. 🧵

submitted 31 days ago • 3 comments

Customizing the Structure of a Minimal TIM Barrel to Craft a De Novo Enzyme https://www.biorxiv.org/content/10.1101/2025.01.28.635154v1

submitted 33 days ago • 0 comments

Distal mutations in a designed retro-aldolase alter loop dynamics to shift and accelerate the rate-limiting step https://www.biorxiv.org/content/10.1101/2025.01.26.634918v1

submitted 36 days ago • 0 comments

Bluetorial-A dream and a bit of a nightmare Serving as Editor-in-Chief at Science was fascinating. I greatly enjoyed working with talented and committed editorial, news, graphics, and production staff. But the inside look into scientific publishing and AAAS was also deeply disillusioning.

submitted 41 days ago • 19 comments

Looking forward to The Protein Society's 39th Annual Symposium! Honored to co-chair the program planning committee with Margie Sunde, alongside committee stars @clvizcarra.bsky.social and Gary Zhang. Explore the program here: www.proteinsociety.org/program

submitted 45 days ago • 0 comments

Mireia Solà Colom gave an incredible seminar at @bpdmc.bsky.social last week, and I'm very excited for her to share some details about what we've built at @aiproteins.bsky.social and how well our #miniproteins perform as therapeutics

submitted 76 days ago • 0 comments

There is no "committee on science" that will impose a new order or set of incentives. No one is going to "fix" peer review or incentivize it differently in one fell swoop. We have to BE the change - the tools already exist for everyone at every level to make an impact and change the incentives

submitted 79 days ago • 3 comments

📣 Save the dates 📅 We are organizing a Benzon Symposium on "Protein structure prediction and design" with what I think is an amazing set of speakers Meeting will take place in Copenhagen 🇩🇰 on Sept. 1–4, 2025, and abstract submission will open in March (benzon-foundation.dk/benzon-sympo...)

submitted 80 days ago • 1 comment

Many industrially-important reactions (e.g. lipase-catalyzed biofuel production) involve substrates poorly soluble in water. How can we screen enzymes for these reactions? Samuel Thompson invented a new 'triple emulsion' platform for biphasic screening (1/n): onlinelibrary.wiley.com/doi/full/10....

submitted 81 days ago • 1 comment

Please share this PhD opportunity in my new lab in Bristol, using protein design/ engineering to understand and build molecular motors to control intracellular transport. Working with @markdodding.bsky.social and Prof Dek Woolfson. www.findaphd.com/phds/project... www.swbio.ac.uk/biomolecular...

submitted 95 days ago • 3 comments

🚨Announcing NetSci: a super fast tool to compute correlated motion in biomolecules pubs.acs.org/doi/10.1021/... @pabloarantes.bsky.social & team also made it into a Colab notebook: colab.research.google.com/drive/1GGJKr... Give it a try & send feedback!

submitted 105 days ago • 5 comments

Happy to announce our latest article on #enzymedesign, in collaboration with Mike Thompson's group! Check it out in JACS: Design of Efficient Artificial Enzymes Using Crystallographically Enhanced Conformational Sampling pubs.acs.org/doi/full/10....

submitted 340 days ago • 0 comments

go.bsky.app/BoXpqQp Computational protein design starter pack. Let me know if I missed you!

submitted 106 days ago • 18 comments

Happy to announce our latest article on #enzymedesign, in collaboration with Mike Thompson's group! Check it out in JACS: Design of Efficient Artificial Enzymes Using Crystallographically Enhanced Conformational Sampling pubs.acs.org/doi/full/10....

submitted 340 days ago • 0 comments

Protein Engineering Canada Conference. 1 week left to benefit from early-bird discount on registration! event.fourwaves.com/pec2024/pages

submitted 343 days ago • 0 comments

Registration for PEC2024 is now open! Come join us this summer in Toronto! event.fourwaves.com/pec2024/pages

submitted 396 days ago • 0 comments

Save the date! The 5th Protein Engineering Canada Conference will take place at the University of Toronto on June 26-28, 2024. event.fourwaves.com/pec2024

submitted 403 days ago • 0 comments

2️⃣ Maud Leonora Menten 🇨🇦 (1879-1960) #womeninSTEM Canadian biochemist, known for her work in enzymology. With Michaelis, she developed an equation that explain the rate of reversible enzymatic reactions & substrate concentration, the Michaelis-Menten equation definingmomentscanada.ca/insulin100/s...

submitted 500 days ago • 0 comments

Save the date! The 5th Protein Engineering Canada Conference will take place at the University of Toronto on June 26-28, 2024. For more info: event.fourwaves.com/pec2024

submitted 483 days ago • 0 comments

“Design of efficient artificial enzymes using crystallographically-enhanced conformational sampling” 🧪🧶🧬 Engineering a Kemp eliminase w/ 250-fold increase in enzymatic activity using an experimental structural ensemble and molecular dynamics-based filtering www.biorxiv.org/content/10.1...

submitted 486 days ago • 1 comment

Happy to share our latest preprint, in collaboration with Mike Thompson's group (UC Merced). Design of efficient artificial enzymes using crystallographically-enhanced conformational sampling www.biorxiv.org/content/10.1...

submitted 486 days ago • 1 comment

Happy to announce our latest article! Here, we report a computational method for rationally tuning enzyme conformational landscapes to tailor catalytic function. In collaboration with Mike Thompson (UC Merced) and Natalie Goto (U. Ottawa). www.nature.com/articles/s41...

submitted 502 days ago • 0 comments