dabiophysicist.bsky.social
Postdoctoral scholar in Veesler Lab @ UWa.
Former UCSF Biophysics & SFSU & UCSC Biochemistry student & Exploratorium Explainer.
I take tiny pictures, in 2 & 3 dimensions.
https://github.com/asarnow
572 posts
1,148 followers
953 following
Active Commenter
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But lawful or chaotic neutral IMO (arguably follows fixed principles)
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I think that really depends on how you define evil and how you handle alignment generally. Just like IRL, (N)PC interests can often be more motivating than ideology
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It does cause some friction when two PCs suddenly sacrifice a third to their lizard god, I have found
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My experience hasn't been (mis)use of these terms as generic, but that trainees can be afraid to apply them to their own observations at all. Other side of the coin maybe
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Btw didn't mean that it's obvious, it wasn't to me for a long time
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That's true, but IMO only a little guidance is needed for newer DM's: decide how often you want them to level and then derive your rewards from the leveling table
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The first rule is "it's your game," after all
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IOW my friend took advantage of player reliance on the DM as sole source of reality to move us organically through a perceptual arc that reversed everything we thought we knew about his world
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Eventually we were fully inducted into underdweller society, converted to their religion, and actively challenged the aboveground ruling caste for control
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As we had more and more contact with them we were forced to understand (through DM storytelling) that they were human, had an ancient civilization superior to ours in many ways, and that they worshiped the land's actual traditional pantheon
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In one of my all-time favorite campaigns, we began as servants of a totalitarian theocracy fighting "underdwellers" who the DM originally portrayed as subhuman goblin-type creatures
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"Forbid" is a strange autocorrect from "encourage," but based on the replies it seems people got the right message anyway
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Terrifying, thank you for sharing that! You might be a little interested to know the newer Jynneos MVA vaccine doesn't generally leave a "take" (I received it in the past couple years, to do poxvirus research)
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Historically "inoculation" can refer both to "variolation" using pox scabs or pustules, widespread in Europe by the 17th century & practiced elsewhere for hundreds of years, or to "vaccination," developed by Jenner & others in the late 18th century using horsepox/cowpox (vaccinia virus)
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FYI the names are confusing and chickenpox (varicella zoster) is a herpes virus, not a pox virus. Very serious in adults as you say, glad your experience wasn't even worse! Both viruses can cause severe illness & blindness, but the 90% naive case fatality rate of smallpox is almost unimaginable now
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What misuse(s) of allostery do you encounter/have in mind?
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Have you tried using spheres instead of surfaces, with a large custom radius, and turning off silhouettes? (ChimeraX silhouettes being unnecessary for Illus/Inkscape, but if you want the outer one anyway you can increase depthJump)
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In mammals at least you can even get an intact site all in one go, from small exons containing ~only a FCS. The obligate FCS in (Sonic) Hh exporter DISP1 for example, where the fly protein has an extended loop instead. Easy to see why FCS insertion & loop truncation might have been favored there.
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You get one of the 82 backup controllers
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IMO x2go is probably an easier option for most than setting up VirtualGL from scratch
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I compiled Relion on my Mac and copy minimal files to run Napari steps locally, however most rejected images are higher tilt/dose and can be excluded when extracting particle tilt series or pseudosubtomograms using the GUI options
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Metareview
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There also seems to be protection even for people vaccinated *after* having HPV disease (neoplasms)
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It really is incredible the extent to which its propensity to emit insane falsehoods stands out in a field of other generally unreliable LLM products
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The Exploratorium also has a very macroscopic exhibit demonstrating the photoelectric effect, and there's a DIY "science snack" version
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The melting point of D2O (3.8˚C) is a good example, and an even better chance to name-drop JD Bernal
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It's also a component of the ethics coursework the NIH requires for PhD students
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Not just how/what we do but also what we *can* do in practice or in theory. I think people tend to have a much more reasonable idea of what's possible in, say, aerospace engineering than in biology
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Moreover spasmodic dysphonia is likely caused by childhood measles infection
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but drug resistant this time
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iow your code is well-commented
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Most of my purifications
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Maybe, but for professional communities it's about moderation. You can't have public scientific discussions that include trainees if your cool microscopy or paper tutorials show up alongside cartoons advocating trans suicide (real example & why I moved)
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Background might be worse for overnight treatment? I've only done the normal way or fluorescent primaries though
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There aren't enough of them in circulation
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they'll probably just take the first marshmallow back actually, you should eat it before they get the chance
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Light exposure really does ruin unsupplemented media. I don't know how long it takes the reducing vitamins to oxidize at 4C
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These viruses evolve rapidly, but we have found 4 different viruses in bats 96-97% identical to SARS-CoV-2 & already possessing expanded host ranges including human...they are actually slightly closer to SC2 than any of the SARS-CoV-1 related viruses eventually found in bats are to SC1
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Instead of competition for excellence, it's competition because there isn't enough to go around
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When awards are so few, many applications of equal quality must be rejected & selections are necessarily decided by chance and personal bias
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Even domestic auto manufacturers can only practically benefit from carefully focused trade restrictions that don't impact supply chains or trigger retaliation
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Who is unionized now? Public sector workers, academic researchers, healthcare workers, educators, manufacturers with complex supply chains (cars), local service professionals (plumbers, electricians), port workers, teamsters, ag workers...
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Losing both is definitely on the table
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Spasmodic dysphonia is likely caused by the measles...
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Parable of a dead salmon
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Pinkerton is still in business too, a subsidiary of Securitas
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Please don't try to collect a tilt pair, I am flying from SFO this weekend
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For the uni as a whole, we're a cost center and if they give up the benefits of hosting us they're actually going to *save* money from the tuition & donor streams that they control
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Fwiw I appreciate the difficulty & imo funding humanities is as critical as my fields of viral & dev bio! I think you're mostly thinking of what would be direct costs, but both categories are windfalls for me, our students, or our program admin and not for the campus budget