diarmuidkenny.bsky.social
Proteomics group leader..... Scale modelling when I can
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588 followers
388 following
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I'm planning on running an olink pilot study so I'll need to get up to speed in this. I see the Coons Lab did a comparison
www.biorxiv.org/content/10.1...
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Am I being silly, but I didn't think somalogic used antibodies. I thought they used protein binding DNA?
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Also consider double digestion. I'm currently running where a specific C is being targeted. The only way we could detect it was digesting with Trypsin and AspN together
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The underside is usually pretty boring; designed to disappear into the sky; maybe painted with sky grey etc and that's all you would see hung up.
I have started to try and give them away to people. If you are ever in Cambridge, UK I can hook you up 😉
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And more competition can only help in driving down costs
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I kind of used Bens blog as my 'bot' to be honest. Its curated and i really just don't have the time between work, two young kids etc to read as much as I would like
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They were also offering a Lumos at deep discount as well
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Thanks. Looks great by the way 👏
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I have heard it this, but never tried it. Do you dilute the putty?
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They were from an older kit that I found at a modelling show.
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It's not daft, it's asked the same question (I am not a FAIMS expert). Their argument was that choosing only CV is too selective and will not produce results representative of the whole sample. To ensure we are not filtering out certain peptides, we need at least 2 CV's.
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I recently completed one with folded Wings...... Is is very fragile
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Thanks for the link. Think it will be a really good topic for a lit review session
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Linkedin is also connected with my employer (my manager, people I manage, directors ect). I'm definitely so not feel as open for discussion on it. I find it very hard on Twitter to find things in interested in lately so hoping here works.
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We have an Exploris. Instrument time is too limited to run on multiple injections. I want to see if we can run with FAIMS for all our workflows as taking it on/off is inefficient, but the team have said DIA requires two CV voltages
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We are running out with some PRM stuff and we still do TMT. Currently not using it for DIA as we use DIAnn for analysis. My experience had been mixed.
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We had one in Cambridge in October. It was really nice to meet Proteomics/mass spec people in and around Cambridge (though there were some London who also attended) My understanding is that they will be having more in the future
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I'm very close to doing the same. I make scale models and there is a scale model community on X. Otherwise I would be gone completely
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I recognise those instructions. It went pear shaped for my too 😧
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I'm stealing this. Very useful to justify the use of FAIMS as my team don't always use it and I'm left wondering why. Have used FAIMS for PRM's? If so, how was it?
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Typo.... It should read electronic recycling graveyard
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We had two, one broken and one fixed. The broken one was taken as a hostage (and sent to the BBC recycling graveyard) by thermo in exchange for the Vanquish. I still have the one the that works.... Not sure yet what we will do with it.
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Yes. Love it. I can have two loading columns so I can load and wash offline. The nearest I can get to having an evosep 😂
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I guess a decision was made to pool the samples.