erpanahat.bsky.social
Cell Biology; Clinical Proteomics; Biomarker Discovery, Development and Validation
Go Blue!
(My posts don’t represent the opinion of my employer.)
13 posts
74 followers
109 following
Conversation Starter
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Can I upgrade my 480 to Excedion?😜
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I run it at 0.8 uL/min with 50C heating and the pressure is at 600 bar. I also commonly use it at 0.4uL/min, off the top of my head it is at around 350-400 bar. 1000 bar if I run it at 1.3 uL/min.
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Recently got a TQ absolute. Will report back once we start running samples with Ionkey. We mostly run plasma and CSF samples.
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Post-transcriptional regulation and post-translation modifications explains big part of the missing correlation?
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This is indeed quite exciting. Very much looking forward to it.
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Thyroglobulin is a famous one. To be honest, there are not many. Unlike ELISA where GMP quality labs produce kits to ensure assay maintenance, I think it’s challenging to maintain a mass spec protein biomarker in the clinic with the labs being the developer and manufacturers at the same time.
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This podcast episode touched on this point you mentioned open.spotify.com/episode/16Yh...
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Rapizyme is pretty awesome. Highly pure, heat resistant.
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Rapizyme maybe able to replace out trypsin+ProteaseMax to be honest if you want it simple.
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To the last point: This the FDR discussion?🤣