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franbottanelli.bsky.social
Solver of GTPases molecular puzzles | Membranes | CRISPR | STED super-res |Accidental immunologist | Academic mother of 2 cheeky monkeys | Assistant prof at FU Berlin. | Head of the Nanoscale Membrane Dynamics Lab
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Germany be wise tomorrow

Our preprints list is now up on FocalPlane! This week we focus on research that has applied microscopy to answer questions in biology. As always, let us know if there are any recent preprints that you think we should highlight. focalplane.biologists.com/2025/02/21/m...

ICE paper submission 🎉 @petia-adarska.bsky.social

During and after science and fun in Heidelberg for our #TRR186 consortium meeting #toomuchscience? #toomuchfun?

Thanks @franbottanelli.bsky.social for leading this effort! Addition genome editing plasmids are available on our labs addgene page. www.addgene.org/Jens_Schmidt/

Check this out, Frans lab made dozens and dozens of knockins in no time!

Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! arxiv.org/abs/2502.12675 1/n

Who the hell decided to book an early train to Heidelberg for half of the lab???

Note to self: make sure all your talks are uploaded on Dropbox and your laptop before leaving for a work trip 😭

If you are having a bad day today think about the guy that was eaten and spit out by a whale. Have a nice weekend all and remember your #worklifebalance

I am excited to share our first Pt replica EM images. It took us a little while, but now we have establish the unroofing, drying and Pt coating workflow 🎉 Great work by our postdoc Luis Wong Dilworth! The image below shows the cytosolic membrane leaflet of a fibroblast 👇

Finally official! We got a DFG-NSF award to fund our collaborative project with @boulantlab.bsky.social at the University of Florida to understand the role of our newly characterized ARF1 compartments in polarized sorting in epithelial cells 🎉🎉🎉 very excited to get started with this!

Are there preprint servers for protocol articles?

I loved the red-green cow on old Twitter. Muuu ing all the red-green non color blind friendly microscopy images. Somebody should create a relocation encouragement cow and muuuu at all the scientists on Twitter/X so they finally stop it and come and hang here.

VPS41 recruits biosynthetic LAMP-positive vesicles through interaction with Arl8b. New study from Paolo Sanzà, Judith Klumperman @judithklumperman.bsky.social and colleagues @umcutrecht.bsky.social: https://buff.ly/4guSZ4H

So many people still posting on the other place. I would love to know why…

Tremendous effort and great native organellar interactome resource from Miguel and co in @harperlabhms.bsky.social : a crosslinking-MS, co-fractionation, and alphaFold interactome on isolated EEA1+ endosomes (+discovery/validation!) | www.biorxiv.org/content/10.1...

The bike commute to visit @andrewplested.bsky.social @benjulab.bsky.social and co on the Humboldt/Charité campus is rather beautiful and right through all the sites ☺️

Ardem, I am sorry. In this middle of this chaos, HHMI will always hold the dubious distinction of being the first organization, federal or private, to cut an ongoing and funded DEI program. In the darkest hour, it ran for the hills. As a member of those groups, I can’t tell you how painful this is.

New paper from the lab🎈. Introducing Micro Immune Response On-chip (MIRO), a device that replicates tumors and their microenvironment to better understand responses to immunotherapies (cyan=immune cells, red=cancer, green=CAFs). www.nature.com/articles/s41... @ibecbarcelona.eu

We're happy to announce the launch of the new website of the Club Exo-endo. From the site, you can register for the annual meeting, register as a new member, advertise a position, apply for the thesis prize or post your latest publication. Please visit us at exoendo.org and make it interactive !

Lots of cool #membrane bio papers in the last few days! Check out my reposts 🤩

Interested how events are coordinated during #endosome maturation? Check out our latest paper! Congrats Daniel, Samit and Jachen! www.embopress.org/doi/full/10....

Protein codes promote selective subcellular compartmentalization | Science www.science.org/doi/10.1126/...