Long in the making but our protocol for gene editing is online! FAB CRISPR is super easy and allows the generation of N and C terminal fusions with TurboID, SNAP, Halo and mStayGold. A single cloninf step to generate HDR templates! http://arxiv.org/abs/2502.12675 1/n
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It took a while for me to understand “problem 4” - perhaps you can write “with an exogenous polyA sequence incorporating the antibiotic resistance cassette”