johannes-uni-wue.bsky.social
4. year, 1. Gen PhD student #UniWürzburg in the Warscheid lab. Phospho-/Proteomics, python, mechanical stress, #TeamMassSpec.
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Astral Zoom, here's the preprint with detailed comparison to the Astral: doi.org/10.1101/2025...
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No gap here.
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But neither you or me did a small mistake with building the source. I don't find another picture online. Could you check please 🙏?
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The trifecta is sitting on my office desk. Love it!
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In the meantime, if anybody needs any assistance currently with their mass spec proteomics project design, method review, data analysis, or result interpretation, please feel free to reach out.. It would be my pleasure to be of assistance.. I am also available for consulting/freelancing.. 5/6
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I wish you a well-deserved break and luck in finding the perfect spot for yourself.
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Just know it from the Astral. But our Ascend or QE are quite stable. Therefore, I just never saw the need for it and was curious. Thank you for the inside.
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In what scenario would you use an internal calibrant?
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It is really dependent on the sample and question I try to answer. For most, I use protein level for imputation as through FDR control and protein grouping the data loses variance.
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Setting the bar high for others 😅
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Do the enriched tissue specific proteins also share similar features or functions?
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The free article for anyone who is now interested in this nice story: www.nature.com/articles/s41...
We are also actively searching for new team members. If you are interested in applying MS methods to study Mitos or Peroxisomes: www.biozentrum.uni-wuerzburg.de/en/biochem2/...
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