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jpassmore.bsky.social
Making cells run in circles, and gels with squares in them. Automated optogenetics, Smart microsopy, Expansion microscopy, GelMap. Postdoc @ Utrecht University | visualise.bio.
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My main PhD paper is on bioRxiv! 🥳 We used motor-PAINT, expansion microscopy, and live-cell imaging of StableMARK to map out how the microtubule cytoskeleton reorganizes during neuronal development. www.biorxiv.org/content/10.1...

submitted 16 days ago • 1 comment

On-Demand Photoactivation of DNA-Based Motor Motion pubs.acs.org/doi/10.1021/...

submitted 25 days ago • 0 comments

Now out in PLOS CB: Spherical Texture extraction! doi.org/10.1371/jour... This method quantifies the intensity distribution in microscopy objects, and is implemented parameter-free in @ilastik-team.bsky.social object classification! We show applications in cells, C. elegans and Drosophila! 😁

submitted 26 days ago • 1 comment

Microscopy Nodes is now up on bioRxiv! 🚀 This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev). High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬 www.biorxiv.org/content/10.1...

submitted 41 days ago • 9 comments

Incredible new work from Yongdeng Zhang demonstrating live 4Pi SIM--and in two colors! www.nature.com/articles/s41...

submitted 63 days ago • 2 comments

Mitochondria consist of networks of cylindrical tubes, right? Not necessarily! - in our new preprint, @gavsturm.bsky.social investigates how mitochondria transiently adopt a beads-on-a-string morphology www.biorxiv.org/content/10.1...

submitted 64 days ago • 6 comments

🍾Our small #UExM adventure is only starting! #ProtistsOnSky We are happy to announce that the Moore Foundation will be funding our efforts in creating an Expansion Microscopy atlas of #Microbial #Eukaryotes with @gautamdey.bsky.social @UNIGE @embl.org LINK: www.unige.ch/sciences/chi... 1/4

submitted 70 days ago • 12 comments

Really bummed to have missed ASCB this year due to some last-minute circumstances, but excited that our manuscript describing an IF disassembly tool will (hopefully) be posted soon. Here's a movie of vimentin color-coded by orientation.

submitted 70 days ago • 1 comment

Advances in microscopy mean we can now do more than just observe biology—we can control it. But how far can we really push this in mammalian cells with all their (beautiful but annoying) heterogeneity? 🧪🔬(🧵)

submitted 70 days ago • 4 comments

OMERO.figure 7.2.0 just released with lots of new features, including "live" interactive inset panels, panel borders, HiLo checkbox, panel flipping, ROI Fill-Colour, ROI Points... Full list at github.com/ome/omero-fi... Thanks to @tom-bssnnt.bsky.social, Rémy Dornier, Tehmina Enayat & Jens Wendt!

submitted 74 days ago • 3 comments

Expansion Microscopy is always amazing to me - here is a dividing U2OS cell expanded ~10X and imaged using a 25X objective! 🔬 A total protein stain using maleimide gives further context. #FluorescenceFriday

submitted 74 days ago • 1 comment

Vimentin DNA-PAINT 🌞 in COS-7 cells #FluorescenceFriday

submitted 81 days ago • 2 comments

🔥 As promised, a thread on our new paper on @biorxivpreprint! First-authored by @icfortunato.bsky.social (experiments) & David Brückner (theory), under the guidance of @ehannezo.bsky.social, @xaviertrepat.bsky.social , and myself www.biorxiv.org/content/10.1... 🧵⬇️ 1/20

submitted 83 days ago • 1 comment

We modified our STED🔬to make it more efficient ('smart') to acquire high-resolution data of small and rare objects in a 3D cell volume. This enabled collecting a large amount of data in a fully automated fashion. With that, we visualized the ulra-structure a small nuclear body, the paraspeckle👇🏻(1/3)

submitted 84 days ago • 1 comment

🎈New preprint! We study how single cells move up, down and about fibronectin gradients. Experiments by @icfortunato.bsky.social, theory by David Bruckner. Collaboration with @raimonsunyer.bsky.social and @ehannezo.bsky.social 👇https://biorxiv.org/cgi/content/short/2024.12.02.626413v1

submitted 85 days ago • 5 comments