kevinstaras.bsky.social
NeuroProf at University of Sussex, UK • Synapses, Circuits, Plasticity, Disease, Decision-making
https://www.thestaraslab.org/
23 posts
235 followers
182 following
Regular Contributor
Active Commenter
comment in response to
post
And here's the brain atlas link again, hopefully working this time: sites.google.com/view/snailbr...
comment in response to
post
sites.google.com/view/snailbr...
comment in response to
post
sites.google.com/view/snailbr...
comment in response to
post
Michael Crossley led the experimental work, supported by Anna Simon, @arndroth.bsky.social and
@enzomarra.bsky.social Thanks to @sussexneuro.bsky.social @leverhulme.bsky.social @ukri.org and @diamondlightsource.bsky.social for funding support. Thanks for reading! 10/10
comment in response to
post
Our approach should readily generalize to other model systems with comparable brain sizes (e.g. other molluscs, crustacea, annelids, insects). On its own, it won’t yield a full wiring diagram, but it does rapidly provide a detailed overview map for atlas building and comparative studies. 9/10
comment in response to
post
This provides the locations of principal feeding-circuit cell types, including motoneurons, CPG neurons and modulatory cells, alongside a detailed summary of their main functional properties. 8/10
comment in response to
post
We also brought together the anatomical mapping and functional information to establish the beginnings of a fully scalable functional cell atlas of the brain of Lymnaea stagnalis: sites.google.com/view/snailbr... 7/10
comment in response to
post
The consistent positioning of neurons across Lymnaea brains means the atlas can guide follow-up functional experiments. Targeting a non-superficial region led to the discovery of DINE (“Diamond Neuron”), an apt name 😜 because it activates the food ingestion circuitry. 6/10
comment in response to
post
The 3D reconstruction revealed the organization of neurons beneath the surface layer for the first time. It turns out around half the neurons (coloured orange) are non-superficial - a hidden world of circuit components that can now be studied. 5/10
comment in response to
post
We then used the excellent volume image-sharing, annotation, and reconstruction platform
@webknossos.org to fully reconstruct the buccal ganglia (one side is shown here) housing the main feeding circuitry, yielding the first accurate estimate of the total number of neurons: ~1100. 4/10
comment in response to
post
Michael Crossley led the experimental work, supported by Anna Simon, @arndroth.bsky.social and @enzomarra.bsky.social Thanks to @sussexneuro.bsky.social @leverhulme.bsky.social @ukri.org and @diamondlightsource.bsky.social for funding support. Thanks for reading! 10/10
comment in response to
post
Our approach should readily generalize to other model systems with comparable brain sizes (e.g. other molluscs, crustacea, annelids, insects). On its own, it won’t yield a full wiring diagram, but it does rapidly provide a detailed overview map for atlas building and comparative studies. 9/10
comment in response to
post
This provides the locations of principal feeding-circuit cell types, including motoneurons, CPG neurons and modulatory cells, alongside a detailed summary of their main functional properties. 8/10
comment in response to
post
We also brought together the anatomical mapping and functional information to establish the beginnings of a fully scalable functional cell atlas of the brain of Lymnaea stagnalis: sites.google.com/view/snailbr... 7/10
comment in response to
post
The consistent positioning of neurons across Lymnaea brains means the atlas can guide follow-up functional experiments. Targeting a non-superficial region led to the discovery of DINE (“Diamond Neuron”), an apt name 😜 because it activates the food ingestion circuitry. 6/10
comment in response to
post
The 3D reconstruction revealed the organization of neurons beneath the surface layer for the first time. It turns out around half the neurons (coloured orange) are non-superficial - a hidden world of circuit components that can now be studied. 5/10
comment in response to
post
We then used the excellent volume image-sharing, annotation, and reconstruction platform @webknossos.org to fully reconstruct the buccal ganglia (one side is shown here) housing the main feeding circuitry, yielding the first accurate estimate of the total number of neurons: ~1100. 4/10
comment in response to
post
A full CNS scan took ~3 mins, and higher-res stacks (voxel size: 0.325 µm) <20 mins. Browse a sample here: wklink.org/2643 3.5/10
comment in response to
post
We plastic-embedded the 3x3x2 mm3 brain and performed X-ray tomography imaging at the Diamond Synchrotron Facility @diamondlightsource.bsky.social
comment in response to
post
The mollusc Lymnaea is a classical system for neural circuit studies. However, we lack a cell-level atlas of its multi-mm scale brain to guide functional investigations. The solution? 2/10
comment in response to
post
This looks like a great resource. Please can you add me - molluscs (and rodents)? Thanks.