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kubasawicki.bsky.social
Bryologist, UWM Sequencing Core Lab Researcher at Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Poland
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Elsevier products: ScienceDirect, Scopus, Mendeley, SciVal, SSRN, Pure, Interfolio. . .and that's not all. "They know what you are working on, they know what you are submitting, they know the results of your peer reviews. They control every part of the process and register every action you take."

1/6 Super excited to share with you our work on Marchantia intra-specific diversity and pan-genomics, just out @naturegenet.bsky.social www.nature.com/articles/s41... Everything on the discoveries in the thread by @chloe-beaulieu.bsky.social I want to emphasize 5 additional points:

Sex chromosime evolution in complex thalloid #liverworts from the perspective of #Riccia genomics. Both Riccia genomes were assembled using @nanoporetech.com Pore-C and native DNA sequencing: nph.onlinelibrary.wiley.com/doi/full/10....

Seriously? 1M$ for Q20 short read machine?

Huge performance update in today’s dorado v0.9.1 release on Ampere 8.6/8.7 & Ada Lovelace 8.9 nvidia GPUs with up to 2x increase in throughput with hac calling and 3x sup! 🚀 Plus a whole bunch of updates for dorado polish and the odd misc bug fix. github.com/nanoporetech...

We are pleased to share our work on the evolution of translation initiation now published in Nature Communications. Take a look at leaderless mRNAs and eS26 in the small ribosomal subunit of Saccharolobus solfataricus. rdcu.be/d5pgg

Mk1 family of @nanoporetech.com sequencers

New kid on the block 😎 Upgrade of our @nanoporetech.com mk1c

The first T2T assembly and annotation of #liverwort Riccia sorocarpa genome using only @nanoporetech.com technology. Done using 2 Promethion flow cells and one minion for cDNA sequencing. More to come soon... www.nature.com/articles/s41...

Some insight into biology and organellar genomics of rare #liverwort Arnellia fennica. Genomic data generates sorely using @nanoporetech.com technology: www.nature.com/articles/s41...

📜 Diverse plant RNAs coat Arabidopsis leaves and are distinct from apoplastic RNAs 🧑‍🔬 @luciaborniego.bsky.social, @msr20.bsky.social, @inneslab.bsky.social, et al. 📔 @pnas.org 🔗 www.pnas.org/doi/10.1073/... #️⃣ #PlantScience #PlantImmunity #PlantRNAs

Adenine DNA methylation associated to transcription is widespread across eukaryotes prelights.biologists.com/highlights/a...

grepq: A Rust application that quickly filters FASTQ files by matching sequences to a set of regular expressions https://www.biorxiv.org/content/10.1101/2025.01.09.632104v1

Dendrograms in ggplot2 heatmaps 🔥 Check out the great `legendry` package by @teunbrand.bsky.social github.com/teunbrand/le...

New Article: "Quantitative RNA pseudouridine maps reveal multilayered translation control through plant rRNA, tRNA and mRNA pseudouridylation" rdcu.be/d5Mrn Comprehensive and quantitative mapping of pseudouridine sites across four plant species. #PlantScience

I wonder if we'll be able to run #dorado @nanoporetech.com basecaller on it: www.nvidia.com/en-us/projec...

Our review is out in Nature Reviews Genetics! rdcu.be/d5AY2 We show how phylogeny-based methods can resolve the problem of non-independence in genomic datasets. These methods must be considered an essential part of the comparative genomics toolkit. @lauriebelch.bsky.social @stuwest.bsky.social

Mumemto: efficient maximal matching across pangenomes https://www.biorxiv.org/content/10.1101/2025.01.05.631388v1

ModiDeC: a multi-RNA modification classifier for direct nanopore sequencing https://www.biorxiv.org/content/10.1101/2025.01.04.631307v1

Really pleased to transition from 2024 to 2025 with Tatiana Vernie's work on the evolution of the common symbiosis pathway published @pnas.org 🔽 We finally demonstrate that ☘️ have maintained a genetic pathway to engage with🍄for half a billion years! www.pnas.org/doi/10.1073/...

Some insights into Ribes organellar genomics with long-reads @nanoporetech.com assembly using single MinION R10.4.1 flow cell: link.springer.com/article/10.1...

I'm going to collect and dry some local Radula species ;) nph.onlinelibrary.wiley.com/doi/10.1111/...

📣 Introducing 📣 microPublications in Ecology and Evolution! Please help support this effort by spreading the word and submitting and reviewing papers! #evolution 🌎 🌍 🌏 🧪 #PlantScience #conservation

Verkko2: Integrating proximity ligation data with long-read De Bruijn graphs for efficient telomere-to-telomere genome assembly, phasing, and scaffolding https://www.biorxiv.org/content/10.1101/2024.12.20.629807v1

A new method for trimming alignments in #phylogenomics — PhyIN. It identifies and trims regions with high phylogenetic discord. Able to trim well even on single loci — an advantage for gene tree/species tree studies. #evolbiol #phylogeny 🧪http://doi.org/10.7717/peerj.18504 Some backstory... 🧵

RNAkinet identifies nascent RNA by predicting 5EU metabolically labeled #RNA from raw #nanopore electrical signal. Should allow association of RNA metabolism dynamics with isoform usage, poly(A) tails and native RNA mods. academic.oup.com/nargab/artic...

Our second paper on hospital wastewater - metagenomics and microbial genomics analysed by @nanoporetech.com R10.4.1 pores: www.sciencedirect.com/science/arti...

The evolution of pectate lyase-like genes across land plants, and their roles in haustorium formation in parasitic plants https://www.biorxiv.org/content/10.1101/2024.12.04.626843v1

Drafted up a Tidyverse-based workflow for micro-synteny. I think it's basic and still effective(?) Made a figure using hypothetical data. I'd like to try some real data before putting it on my GitHub. If you have a favorite locus for micro-synteny, send me a message. #Genomics #DataVisualization

Pod5Viewer: a GUI for inspecting raw nanopore sequencing data. #Nanopore #SequencingData #Bioinformatics #Genomics @nanoporetech.com 🧬 🖥️ academic.oup.com/bioinformati...

Phylogenetic turnover of seed plants 🌱 at a global 🌐 and across phylogenetic scales: www.nature.com/articles/s41...

NCBI's publicly available eukaryotic genome annotation tool, EGAPx: now using orthology and well-supported proteins to classify and name genes, automatic configure based on the species taxon, etc. 🧬🖥️🧪

Also pumped mPCRselect is out! Work was co-led by @elliecat.bsky.social & former undergrad Julie, we added sims from our tech Tessa, & software updates from Michael. mPCRselect can take a VCF & conduct marker selection for indiv. ID and pop assignment onlinelibrary.wiley.com/doi/10.1111/...

Exciting news! The latest hifiasm release from Haoyu Cheng and Heng Li adds beta support for @nanoporetech.com simplex R10 reads. Initial results look very promising. 🚀 Check it out: github.com/chhylp123/hi...

Our lab's latest preprint investigates the dynamic RNA modification landscape in the #liverwort Riccia fluitans during its transition from a terrestrial to an aquatic environment. We employed @nanoporetech.com DRS for the identification and validation (adapted PRAISE metod) these modifications.

In our previous study examining the epitranscriptome of the amphibious #liverwort Riccia fluitans, we performed both SBS RNA-seq and @nanoporetech.com direct RNA sequencing (DRS) using the same RNA extract. bmcplantbiol.biomedcentral.com/articles/10....

The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1