phagemorgan.bsky.social
MD/PhD student. Harm Reductionist. Here for the phages. All views my own.
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I love this.
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Sure but the Juk in PA14 prevents KZ replication. Other juks probably protect against different phage. For example KZ and it's close relative PA3 share a replication mechanism but PA3 is insensitive to PA14 Juk.
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www.biorxiv.org/content/10.1... Discovery of Juk system in PA14 providing resistance to both PhiKZ and OMKO1 www.biorxiv.org/content/10.1... Knockdown of Juk restores PA14 PhiKZ sensitivity pubmed.ncbi.nlm.nih.gov/38963841/ and here we use PA14 to select out PhiKZ for quantification
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I'm not sure what MOI exactly means on a plate. 10^7 phage on a spot assay will make a clearing, but you don't get productive infection. I use PA14 to select against phiKZ during competition experiments because it's more or less completely resistant. I'll drop a few papers in a second.
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How did you get phikz to infect PA14 when it is naturally resistant due to the Juk system? How did you get different results for phikz and omko1 when they are nearly generically identical?