rachaelott.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

High-resolution optical imaging of the meninges within the brain of an adult zebrafish. Credit to @zebrafish007.bsky.social & #WeinsteinLab. #ZebrafishZunday 🧪

submitted 1 day ago • 3 comments

#FlyWeek fun for #FluorescenceFriday, nuclei and α-spectrin are shown in this stack of adipocytes from adult Drosophila 🔬 #Microscopy #CellBiology

submitted 3 days ago • 0 comments

#FlyWeek fun for #FluorescenceFriday, nuclei and α-spectrin are shown in this stack of adipocytes from adult Drosophila 🔬 #Microscopy #CellBiology

submitted 3 days ago • 0 comments

For #FluorescenceFriday, virally labeled projection neurons in the mouse prefrontal cortex that are targeting the locus coeruleus. Image credit to the circuits team in the Translational Neuroscience division @lieberinstitute.bsky.social 🧠🔵🧪

submitted 3 days ago • 2 comments

My #FlyWeek contribution: The Variety of Testis Shapes in Pomace Fly Species Aren't they beatiful?🌈🪰🔬 An old-school sci-poster meets modern confocal imaging! #Drosophila testis diversity fascinated even Curt Stern (yes, the human genetics textbook guy!) back in the 1930s. #microscopy #insects

submitted 4 days ago • 3 comments

You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software! Hope it will be useful !

submitted 6 days ago • 3 comments

Exciting paper by the groups of Ronald Micura and Alexandra Lusser in @nchembio.bsky.social. Covalent binders to structured RNA based on alkyl halides and alkyl mesylates show engagement of the preQ1 and Pepper aptamers in vitro and in cells. www.nature.com/articles/s41...

submitted 124 days ago • 0 comments

For #FluorescenceFriday, actin is shown in a medium spiny neuron 🔬 #Microscopy #Neuroscience

submitted 9 days ago • 1 comment

For #FluorescenceFriday, actin is shown in a medium spiny neuron 🔬 #Microscopy #Neuroscience

submitted 9 days ago • 1 comment

Today a pupal #Drosophila #Brain (40 h APF) for #fluorescencefriday. 🔵 Actin 🟠🟡 Anti-E-Cad depth-coded color I have 1 million versions of this picture, in which I tried different LUTs. I will put some in the comments! #microscopy #cellbio #neuroscience #insect

submitted 10 days ago • 2 comments

My mom used to love #FluorescenceFriday, so I will keep posting in her memory ❤️ This photo shows neurons in the hippocampus accumulating phospho-tau in our monkey model of Alzheimer's Disease. Markers: Dapi 🔵, Neun 🔴, pTau 422🟣, AT8🟡

submitted 10 days ago • 7 comments

New lab preprint! High resolution mapping of the actin fusion focus reveals myosin V-dependent transport of formin for actin aster compaction. A very cool work led by @valthomas.bsky.social and nice collaboration with Andrea Picco and @kaksonen.bsky.social! www.biorxiv.org/content/10.1...

submitted 10 days ago • 2 comments

Postdoc position in my lab: investigating how the microtubule motor protein dynein-2 works in cilia. Here's dynein-2 at work translocating microtubules - how is this activity regulated? How does dynein-2 malfunction in disease? Find out more and apply: my.corehr.com/pls/uoxrecru...

submitted 12 days ago • 2 comments

This time-lapse captures 17 hours of axonal growth from a chicken dorsal root ganglion explant, visualized through the actin cytoskeleton using live confocal imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours! 😉 🧪

submitted 12 days ago • 71 comments

For #FluorescenceFriday, a GFP reporter shows nuclei (fire) nestled between lots of lipid droplets in this set of adipocytes 🔬 #Microscopy #CellBiology

submitted 17 days ago • 0 comments

🚨🔬💗Whether investigating cell organelles or mapping proteins, together with Victor Puelles's lab we lay a roadmap for selecting optimal #ExM and #SuperResolution #microscopy combinations. Daria Aristova and Dominik Kylies review with amazing co-authors pubs.aip.org/aip/apr/arti...

submitted 17 days ago • 0 comments

For #FluorescenceFriday, a GFP reporter shows nuclei (fire) nestled between lots of lipid droplets in this set of adipocytes 🔬 #Microscopy #CellBiology

submitted 17 days ago • 0 comments

For #FluorescenceFriday - pretty image of ventral tegmental area (VTA) in the mouse 🧠 labeled with tyrosine hydroxylase (TH) 🟣. Red and green are from experiments trying to virally label various projection neurons targeting the brainstem and midbrain 🔬🧪

submitted 17 days ago • 3 comments

Happy to share our new publication in ACS Chemical Biology @pubs.acs.org reporting a new chemical biology tool – dual SLIPT! Congratulations to first author Kristina Bayer @XXX, all co-authors and collaborator Shige Yoshimura @XXX. Open Access: pubs.acs.org/doi/10.1021/... (1/5)

submitted 17 days ago • 2 comments

Super happy that our papers #2 and #3 from our 3-person lab just went online on bioRxiv! First large-scale 3D EM dataset from mouse hippocampus CA1 www.biorxiv.org/content/10.1... - check out the beautiful data on webknossos wklink.org/7023 ! …

submitted 19 days ago • 3 comments

WOSM-TIRF up running! Awesome #Tirf imaging of microtubules and EB comets in #Xenopus egg extract. The WOSM-TIRF is the big brother of the eduWOSM (doi.org/10.7554/eLife.104046.1). So grateful to @irobcross.bsky.social & Nick Carter for this collaboration & @agencerecherche.bsky.social for funding.

submitted 20 days ago • 2 comments

A dream come true: the first expansion microscopy images of C. flexa 🤩 Generated by Mylan & Uzuki who learned from the best (@hiralshah.bsky.social @gautamdey.bsky.social @dudinlab.bsky.social). We will learn so much from these!

submitted 20 days ago • 18 comments

Happy to officially introduce FilaBuster - a strategy for rapid, light-mediated intermediate filament disassembly. Compatible with multiple IF types, modular in design, and precise enough to induce localized filament disassembly in live cells. www.biorxiv.org/content/10.1...

submitted 20 days ago • 2 comments

🧫 A gentle reminder that your gut is an actual work of art. #FluorescenceFriday #BeneathTheScope

submitted 24 days ago • 0 comments

For #FluorescenceFriday, tau (cyan) and neurofilament proteins (fire) are shown in these primary hippocampal neurons 🔬 #Neuroscience #Microscopy

submitted 30 days ago • 1 comment

DM1A never disappoints 🤩 #FluorescenceFriday

submitted 30 days ago • 2 comments

For #FluorescenceFriday, tau (cyan) and neurofilament proteins (fire) are shown in these primary hippocampal neurons 🔬 #Neuroscience #Microscopy

submitted 30 days ago • 1 comment

Gills just wanna have #FluorescenceFriday 🎵 Phalloidin reveals the beauty of the filament's distal tip in cleared gills of adult 🦓🐟 #microscopy #devbio #sciart

submitted 31 days ago • 1 comment

Reposting some old pictures for #fluorescencefriday! A pyramidal neuron (violet) & a microglial cell (cyan) labelled with DiI from the prefrontal cortex of a rat brain.

submitted 31 days ago • 2 comments

A culture of boa constrictor cells expressing a fusogenic viral protein, leading to cell fusion and giant multinucleated cells! Actin is in gold, membranes in white and DNA in blue. #FluorescenceFriday #SciArt

submitted 31 days ago • 3 comments

#fluorescencefriday. Let’s take a look at the brain vasculature with this rendered video in a whole mouse brain. 🧠 Labelled with CD31 by @shahrzadbahrampour.bsky.social

submitted 31 days ago • 0 comments

Choroid plexus is often lost during tissue sectioning and overall anatomy can be difficult if not impossible to reconstruct. Here we observe the vascular anatomy of human choroid plexus as if through a jeweler’s loupe. Sample courtesy of Matthew Schrag and Neil Dani. #FluorescenceFriday

submitted 31 days ago • 7 comments

If you ever hear someone say "It feels like my brain is on fire", don't psychologize them. This is what neuroinflammation looks like. Imagine being a red blood cell whose only mission is to deliver oxygen, only to find a river of fire waiting for you inside the brain. #NeuroCovid

submitted 32 days ago • 10 comments

Happy #WidefieldWednesday Bluesky!! A new hashtag to appreciate all of the wonderful widefield #microscopy work shared here 🧪🧠🔬 Today we are sharing this photo of a primary #astrocyte grown on cover glass used in the study of #Parkinsons disease, imaged with a VS200 slide scanning microscope.

submitted 33 days ago • 0 comments

How can organs regenerate fast? Bioelectricity helps -- by coupling sub-second electrical injury signals to regenerative proliferation. First preprint from my potsdoc :D with @liujinghui.bsky.social @ritamateus.bsky.social @mpi-cbg.de and collaborators @mpipks.bsky.social @csbdresden.bsky.social

submitted 33 days ago • 2 comments

🔬 Exciting discovery! We've been curious about the function of unique IBA1+ cell staining we observed around healthy human DRG neurons in the lab (indicated by red arrows). A new paper @cellpress.bsky.social finally provides some answers. Link: www.cell.com/cell/fulltex...

submitted 34 days ago • 1 comment

Important to celebrate trainee victories: excited to have Holly Merta's @hollymerta.bsky.social paper out! We use TurboID sub-organelle proteomics to map the ER network. We find calmin/CLMN, an ER-to-actin tether regulating focal adhesions & cell motility. www.sciencedirect.com/science/arti...

submitted 35 days ago • 3 comments

For #FluorescenceFriday, lipid droplets (orange) and α-spectrin (blue) are shown in adipocytes 🔬 #Microscopy #CellBiology

submitted 38 days ago • 5 comments

For #FluorescenceFriday, some images fresh off the scope for new experiments that I am so extremely excited about. This is staining for tyrosine hydroxylase (TH) in iPSC-derived neurons where we are optimizing a differentiation to make locus coeruleus-like neurons 🔵🔬🧠🧪

submitted 38 days ago • 1 comment

Hi #FluorescenceFriday community! 🌈 Fly testes are unexpectedly stunning 😀 Here’s a testis from a non-melanogaster Drosophila species: 🟠🟡 DNA: Dapi 🔵🟢 Muscles: Phalloidin LUT: @kwolbachia.bsky.social Scope: @nat-prunet.bsky.social 's core #sciaart #microcosm #closeup #microscopy #cellbio

submitted 38 days ago • 4 comments