sciria.bsky.social
HHMI Hanna Gray Fellow at Stanford University in KC Huang's Lab | PhD @ MIT Biology in Grossman Lab | Exploring mechanisms driving horizontal gene transfer in the human gut microbiome | I love anything outdoors, good food, and craft cocktails | 🏳️🌈🍸🥘⛺
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doi.org/10.1371/jour...
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If you're excited about MGEs and the transference of this type of perspective to the gut microbiome, I hope to update everyone soon on some work I've been doing regarding HGT in the mammalian gut. Here's a snapshot of things to come.
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And 2) show that the timing of integration in transconjugants is critically important and links two essential functions of the ICE lifecycle: replication and integration.
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In the paper, we delve a bit into why MGEs may have evolved these functions. If interested in more work like this, please check out some of my other publications where 1) we characterize how an integrative and conjugative element finds its integration site:
doi.org/10.1093/nar/...
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Though not fully elucidated here, this seems to differ from the mechanism utilized by PsiB encoded by F-plasmid as it is active in donors and is not believed to interact directly with RecA. Alam Heredia has done excellent follow-up characterizing the mechanism of action here: doi.org/10.1101/2024...
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We show that ICEBs1 in Bacillus subtilis encodes genes that inhibit RecA filament formation. This prevents activation of co-resident prophages following a DNA damage event and inhibits ICEBs1-mediated activation of the SOS response during rolling-circle replication.
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🙋🏽♀️
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🙋🏽♀️
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🙋🏽♀️
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🙋🏽♀️
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Add me please! 🙏🏽
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Congrats!! 🥳🎉🥂