swaminathanlab.bsky.social
Laboratory of Cell and Molecular Mechanobiology at Lund University, Sweden
Interested in all things integrins, adhesions, cytoskeleton, microscopy
29 posts
212 followers
174 following
Regular Contributor
Conversation Starter
comment in response to
post
as usual beautiful data. big congrats!
comment in response to
post
Holy wow-- this is amazing!! Congratulations!
comment in response to
post
what a fantastic lineup!!
comment in response to
post
This work has led us now into a whole range of new projects and collaborations that we are very excited about. This project was a collaboration with the groups of Adam Simonsen at SDU, Cathy Merry and Amanda Wright at Nottingham and Pontus Nordenfelt
comment in response to
post
This work was inspired and stands on the shoulder of amazing work from several labs that have been developing alginate based hydrogel system and/or have been working on ECM stiffness-mechanotransduction pathways in epithelial structures for decades
comment in response to
post
Mechanistically, using antibodies, inhibitors etc, Kabilan found that this process of in-situ stiffening induced ECM remodelling and invasion of normal mammary acini was driven by beta1 integrin-FAK-Piezo1 pathway with Piezo1 specifically being downstream of integrin activation and signalling.
comment in response to
post
Kabilan then followed the process of in-situ stiffening induced changes in acini morphology and ECM properties over time and found interesting and "dynamic" changes in relative ECM enrichment which coincided with different stages of growth and the onset of invasion
comment in response to
post
Interestingly, he found several differences in basement membrane and ECM(Fibronectin) enrichment and organisation between acini embedded in in-situ stiffened gels compared to acini formed from single MCF10A cells embedded in stiff gels from Day 1 (see below)
comment in response to
post
Here Kabilan first embedded single MCF10A cells in a soft interpenetrating network hydrogel system and allowed it to form a normal mammary acini with its own native ECM environment. He then in-situ stiffened these gels thus mimicking tumour growth and invasion on normal 3D structures-
comment in response to
post
this is beautiful. Fantastic work.--
comment in response to
post
In all seriousness,use CiteAb and Antibodypedia, look for KO/KD validated antibodies, best - recombinant, second best - monoclonal, and if possible do your own KO/KD controls, or induction by ligand/small molecule,if not - compare hyperexpressing with low expressing cells (from depmap/protein atlas)
comment in response to
post
I would win this if falling asleep in the room is an allowed strategy.
comment in response to
post
comment in response to
post
There is lots more to tell about the university, the centre, the people and the country. Contact me if you have any questions.
comment in response to
post
The town boasts 2 giant science infrastructures including MAX IV which is the world's first 4th generation synchrotron light source facility and soon to open, the European Spallation Source ERIC (ESS)
en.wikipedia.org/wiki/Europea...
comment in response to
post
Lund is a fantastic college town 10-15 mins from Malmö and 30-40 minutes from Copenhagen airport. Its home to several biomedical and tech companies, excellent schools, a fantastic university and just incredible diverse people
en.wikipedia.org/wiki/Lund
comment in response to
post
Wallenberg centre for Molecular medicine(WCMM) is an initiative funded by the Knut and Alice Wallenberg Foundation. The link to WCMM Lund is:
www.wcmm.lu.se/wallenberg-c...
comment in response to
post
Thanks @guijacquemet.bsky.social - This really made it easy to transition!