shinyatsukiji.bsky.social
Chemist | PI of the Tsukiji Lab at Nagoya Institute of Technology | Pursuing new molecular concepts and tools for chemical biology, cell biology, and synthetic biology
http://tsukijilab.web.nitech.ac.jp/index-e.html
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Finally out! Congratulations, Zhixing! 🎉
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🎉 Congratulations! Great work and thank you for extending our SLIPT technology! I just met up with Yoshimura-san yesterday and had a great time chatting!
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Very cool work! Congratulations, Michelle! 🎉
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Awesome work! Congratulations! 🎉
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This is an awesome collaboration with Hiroshi Murakami & his team (Nagoya U)! Huge shoutout to our amazing co-first authors Tomoki Miyazaki & Tomoshige Fujino, who led the project. And big thanks to Tatsu Yoshii for his key contributions early on. Grateful to all the authors!
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Beyond the practical utility of AzoTag tools, this strategy opens exciting new avenues for the de novo development of tailored pairs of synthetic photo-responsive small molecules and artificial protein binders for optical manipulation of biomolecular functions.
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The in vitro-selected AzoTags work in mammalian cells! Together with HaloTag, they enable precise, reversible, and repeatable switching of cellular processes using dual light. Several applications are showcased in the manuscript.
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Using this approach, we created artificial proteins named AzoTags, which selectively bind to the cis-forms of designed azobenzene derivatives. The trans–cis isomerization of these molecules can be reversibly controlled using two distinct wavelengths of visible light.
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In this work, we present a novel strategy that combines the rational design of synthetic photo-switchable molecules with the in vitro selection of artificial protein binders—via TRAP display, an advanced mRNA display—that specifically recognize a particular conformation of these molecules.
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Chemo-optogenetics, which uses synthetic photo-responsive ligand–protein tag pairs, offers a promising alternative. However, redesigning existing small-molecule ligands for protein tags into photo-responsive versions with desired properties is also difficult.
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Optical control of biomolecules is a powerful technique in biological research, but current optogenetic tools derived from natural photoreceptor proteins face various limitations. Reengineering these proteins to meet specific research needs remains a major challenge.
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The in vitro-selected AzoTags function in mammalian cells! In combination with HaloTag, they enable precise, reversible, and repeatable switching of cellular processes using dual light. Several applications are showcased in the manuscript.
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Using this approach, we generated artificial proteins called AzoTags, which selectively bind to the cis-form of a designed azobenzene derivative. The trans–cis isomerization of this molecule can be reversibly controlled using two distinct wavelengths of visible light.
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In this work, we present a novel strategy that integrates the rational design of synthetic photo-switchable molecules with the in vitro selection of artificial protein binders that specifically recognize a particular conformation of these molecules.
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Optical control of biomolecules is a powerful technique in biological research, but current tools derived from natural proteins face significant limitations. Tuning these proteins to meet specific research needs remains a challenge.
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Congratulations, Jeremy! 🎉
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Thank you Zhixing! Looking forward to meeting you in March!
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March 27 (Thu), 13:00–15:40
Speakers: Yukihiro Itoh (Osaka Univ.), @junseoklee.bsky.social sky.social (Korea Univ.), Haruo Aikawa (Univ. of Tokyo), Gong Chen (Nankai Univ.), Minami Odagi (Tokyo Univ. Agri.), Tuoping Luo (Peking Univ.)
pub.confit.atlas.jp/en/event/csj...
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March 27 (Thu), 9:00–11:40
Speakers: Hiroyoshi Fujioka (Inst. Sci. Tokyo), @zhixingchen2.bsky.social (Peking Univ.), Satoshi Okada (Inst. Sci. Tokyo), Fan Zhang (Fudan Univ.), Hao Zhu (Kyoto Univ.), Chu Wang (Peking Univ.)
pub.confit.atlas.jp/en/event/csj...
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Program Overview
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This is the original paper👇
pubs.acs.org/doi/10.1021/...