gnevot.xyz
Postdoc at Marc Güell's lab at UPF Barcelona | Engineering the microbiome to understand (and treat) the skin 🦠 #SynBio | Alumni LPI (former CRI) & UniZar | 🇪🇸 🇫🇷 🇬🇧
More info: www.gnevot.xyz
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Excited to see this projecte developing so greatly! We published an interview with Javier Santos a couple of years ago about the #skindev kick-off ellipse.prbb.org/skindev-a-sy... and are very proud and happy to see how far it's gone! Congrats @gnevot.xyz and all those involved!
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If you reached until here I guess you are as excited about C. acnes as we are! All our plasmids are available in @addgene.bsky.social and @sevaplasmids.bsky.social for you to play. We are also happy to collaborate to advance C. acnes! All the info in our paper👇
authors.elsevier.com/sd/article/S...
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Here is a video of our #SKINDEV project if you want to know more of what we are cooking in the background 😉 www.youtube.com/watch?v=RvhT...
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Also thanks to our great skindev collaborators #EllenBogaard #PatrickJansen from @radboudumc.bsky.social and #IçvaraBarbier #RodrigoLedesma from @imperialcollegeldn.bsky.social
. Also, thanks to our great editor #ErnestoAndrianantoandro for all his enthusiasm and guidance over the publication.
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Of course, this work would not have been possible without the funding from the @ec.europa.eu #EIT #SKINDEV, the mentorship of @marcguellc.bsky.social and the efforts from the team at #SynbioLabUPF @upf.edu @prbb.org with special thanks to #JavierSantos!)...
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Very nice Guille, so what’s next? We hope to have demonstrated the potential of C. acnes to become the best microbial chassis for synbio applications in the skin and we are working to validate this in more realistic models so stray tuned!
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For therapeutics, we implemented a UV-damage kreatinocyte model and see if the supernatant of an engineered C. acnes producing a recombinant antioxidant protein could protect the cells. Turns out it reduced significantly the ROS levels in the UV exposed keratinocytes!
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For biocontainment, we focused on targeting key metabolic genes so C. acnes would only survive if we topically supplement the missing amino acid. Since knocking out genes is still challenging in this skin non-model bacterium, we turned to #CRISPRi to quickly screen auxotrophs.
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How did we apply these tools? We focused mainly on two objectives:
1️⃣ Biocontainment strategies for safely deploying C. acnes in human skin 🛑🦠
2️⃣ A proof-of-concept therapy to combat UV-induced oxidative damage ☀️💥
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Thus, we have focused in engineering C. acnes. Now, we offer a whole set of tools for making C. acnes smarter with:
🔨 Modular and optimized plasmid
🔨 Basic expression tools: promoters, RBSs, terminators…
🔨 CRISPRi
🔨 Natural and synthetic inducible systems
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Although C. acnes has a bad rep bc *some strains* are associated with acne, it’s actually the most abundant #skin commensal for #synbio:
✅ Colonizes the skin follicle where the skin is the thinnest
✅ Low turnover
✅ Genetically stable
✅ Engrafts after topical application
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For mine (gnevot.xyz) I use Notion with NoteHost with free hosting in cloudfare. Supereasy to modify and customize, just need to figure out positioning in google searches
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Accurate RNA 3D structure prediction using a language model-based deep learning approach
www.nature.com/articles/s41...
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I'd be great to join!
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Would love to be included 😊