heikelindner.bsky.social
Plant Developmental Biologist @unibern, interested in leaf development, Kalanchoë, CAM
Mindful Parenting Advocate, Feminist, Mami, Synesthete, she/her
31 posts
164 followers
160 following
Regular Contributor
Active Commenter
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Aw, that‘s so sweet!! You deserve it! Congratulations Kutscher lab!
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Congratulations to you and your team! Another amazing story about hydropatterning 💪
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Congratulations Karina! Well deserved 💪
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Ich denke heute ganz besonders an Euch ❤️
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Congrats Jamie et al! Looks really interesting!
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Yes! Thank you!!
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The link somehow doesn‘t work.
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Thank you José! This kind feedback means a lot to me!
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Together, we propose that much like in grasses, Kalanchoë MUTE makes stomatal subsidiary cells by delaying the guard cell program and inducing an asymmetric division program. 9/n
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Finally, we used transcriptomics to detect the deregulated downstream genes when Kalanchoë MUTE is overexpressed. Unlike in Arabidopsis, genes linked to asymmetric divisions are upregulated and genes linked to guard cell differentiation are downregulated. 8/n
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This suggested that MUTE in the leaf succulent Kalanchoë makes subsidiary cells. Strikingly, this is more similar to MUTE’s role in the distantly related grasses than in the more closely related Arabidopsis. 7/n
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When overexpressing Kalanchoë MUTE, many additional subsidiary-cell-like cells were formed in Kalanchoë. This is strikingly different from the MUTE overexpression phenotype in Arabidopsis, where many additional guard cells pairs rather than subsidiary cells are formed. 6/n
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Indeed, fluorescent reporter genes showed that both MUTE homologs are expressed specifically during the asymmetric divisions forming SCs. This suggested that MUTE might be involved in controlling SC divisions. 5/n
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Gene-editing the two homologs of the core stomatal transcription factor MUTE disrupted stomatal formation and messed up asymmetric divisions - a first hint that Kalanchoë MUTE has a different role in leaf succulents than Arabidopsis. 4/n
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10-day time-lapse imaging showed that young precursor cells undergo 6(!) rounds of asymmetric division oriented in a Fibonacci spiral. The first three divisions make non-stomatal cells before divisions 4 to 6 make the 3 subsidiary cells. Finally, a symmetric division makes the guard cell pair. 3/n
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Unlike in Arabidopis, Kalanchoë stomata consist of two guard cells and three circularly arranged subsidiary cells. K+ staining of open and closed stomata shows that K+ is shuttled between the two stomatal cell types. Therefore, Kalanchoë’s subsidiary cells are likely functional helper cells. 2/n
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Unfortunately, we haven‘t done it, yet. But it would be super interesting!
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What an awesome resource 🤩
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K. laxiflora makes plantlets if you detach the leaves from the mother plant. In daigremontiana the LEC gene is mutated so it doesn‘t produce viable seeds. Kalanchoë is such a fascinating genus 😍
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Wow Ora! Congratulations on your new position but what a loss for Swiss Plant science!
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Nur durch Dozenten wie Dich versteht man Sachverhalte genau, weil man nicht abnickt um nicht dumm da zu stehen. Ich wünschte mir mehr Lehrende mit so viel Passion. Und ich wünsche mir mehr Anerkennung für Eure Leistungen!
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Herzlichen Glückwunsch lieber Roland! Das ist mehr als verdient! Deine Lehrveranstaltungen sind lehrreich, durchdacht und unterhaltsam. Was ich jedoch immer am meisten geschätzt habe: Du hast nie ein schlechtes Gefühl vermittelt wenn man etwas nicht verstanden hat, sondern mit viel Geduld erklärt 💪
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Wow, this bag!! 😍
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We have a masters program at the University of Bern. Courses are not only plant-related, but her thesis could be in our lab working on Kalanchoe or Brachy stomatal development 🪴
www.philnat.unibe.ch/studies/stud...
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Good luck 🍀
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Congratulations @duartedf.bsky.social ! Very well deserved! Sounds like a super interesting project 💪
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Congratulations @plantophagy.bsky.social !! They are so lucky to have you! All the best for you, your family and your lab! Wishing you a smooth transition.