mishakudryashev.bsky.social
Scientist at the Max Delbruck Center for Molecular Medicine @mdc-berlin.bsky.social in Berlin.
Professor in Charité – Universitätsmedizin Berlin
Biophysics, data and structural biology at its coolest
20 posts
433 followers
182 following
Getting Started
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Thanks Andrew! this is a beautiful paper from @johnrubinstein.bsky.social, they are much better in getting high res structures! We posted preprints roughly at the same time and it took us a bit longer to publish, their paper came out first along with: www.nature.com/articles/s41...
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They would have told you but it's against GDPR !!
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😍❄️🔬🥬
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Thanks Michaela and Hallo World! :)
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Excellent perspective Juan!
technically many packages are open-source, such as tomoBEAR!
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thanks! I am happy that you liked it! You have also been there probably with some vintage microscopes !?
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Now I have the first author Adria @adriasogues.bsky.social as a bluesky friend!!
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Thank you very much, he is absolutely terrible!
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S-layer is a very advanced protective layer, optimized by evolution, and understanding its structure is highly important for its potential destruction.
It was a team effort and we thank our institutes, colleagues, and funders!
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Adria Sourges expressed and co-crystallized the combinations of the domains to get the structures of the interfaces, which finally allowed us to build the correct atomic model, which was validated by mutations. A single mutation at the D2-D2 interface can break the lattice
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It is a nice structure but the resolution was not enough to place 6 very similar domains into it.
Alex Pak from Colorado performed MD on several of the possible arrangements, checking their stability over time
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But it was only a partial construct and in the real bacteria, they form a lattice. Sander Van Der Verren produced pretty tubes, which Kendra Leigh imaged with cryo-ET and got a 7 Å structure by subtomogram averaging.
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S-layer acts as a mechanical exoskeleton, crucial for B. anthracis stability and survival under stress. Antonella Floravanti, Han Remaut and Co previously reported an X-ray structure that gave insights into the therapeutic intervention mechanism
www.nature.com/articles/s41...
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I am new here, feeling the vibes !! Thanks for the thumbs up Alexis !!