sarajweaver.bsky.social
Structural biologist in drug discovery. #CryoEM specialist. #MicroED. X-ray #crystallography. #teamtomo Industry.
4 posts
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300 following
Prolific Poster
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- identifies crucial contacts; very useful for selecting mutagenesis targets
- fixes model geometry. In cryo-EM, stereochemistry is usually the best priors you have!
- helps predicting models of weak complexes that AlphaFold3 cannot
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Our findings emphasize how we use PDB models. It is critical to understand that PDB models are just that, models. They do not fully explain the underlying experimental data, have nuances in how they are representing the data, and as we show, can vary in how well the underlying data is explained.
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Here is a CryoEM starter pack I try to assemble
go.bsky.app/JmQksC
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#7 www.biorxiv.org/content/10.1...
- Nice logical flow of cryoEM images at different angles side-by-side with the model
- I like that the pointer lines don't overlap with the structures and all drawn at 90 degree angles
- I like the design on the view rotation symbol. Never seen one look like that!
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just dropping back in to add this blog about Google's DeepMind's effect on the field of Protein Structure Prediction, and scientist's reaction to the opacity of their methods in a field where everyone usually shares everything to further the science moalquraishi.wordpress.com/2020/12/08/a...
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Finally, required DALL·E 3 abstract summary...
(Prompt: protein structure drawn in cartoon mode colored in rainbow, surrounded by small molecules resembling amino acid sidechains colored grey)
Looks a little like spaghetti, 🍝 but given one of the coauthors is Italian, i figured... 😅 (10/10)
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Read all about it! All feedback is welcome. Please share when the method fails! We are actively working on improving and debugging. 😀https://biorxiv.org/content/10.1101/2023.10.15.562410v1… (9/10)
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The #OpenSoftwareAcceleratesScience code can already be downloaded from the link below. However, it may be worth waiting another week or so, when we release #RELION5 for easier installation and full integration in its GUI.
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I think they were order earlier in pandemic when it was hard to get a hold of anything. So you order a few from everywhere and 6 months later you get way too many grids. I certainly wouldn’t order this many normally. Usually I just make them in lab.