Microscopy peeps: If you could teach 1 thing to users, what would it be?
For example: geometrical optics/aberrations/RI theory? maybe some electrical thing? how lasers work? where vibrations come from? Why images are blurry?
For example: geometrical optics/aberrations/RI theory? maybe some electrical thing? how lasers work? where vibrations come from? Why images are blurry?
Comments
& the importance of cleaning up (oil, lens wipes, slides) when you finish.
Saturation and bleaching, and how to not let either wreck your experiments.
PSFs and deconvolution.
Bit depth.
I think the relationship between pixel size and resolution and resolving a certain size object is a good start.
Often just explaining what a pixel and a histogram is goes a long way.
I suppose next would be properties of fluors.
Or...
When it comes to immersion oil... less is more
I love the "part of microscope" phrase. Your embedding matters!