Let's share some other classic, the beautiful Cells in culture film directed by Michael Abercrombie one of the father of quantitative cell imaging (University College London, 1955) is available here identity.wellcomecollection.org/works/z6h7jzv4 - ThreadSky

guijacquemet.bsky.social • 109 days ago

Let's share some other classic, the beautiful Cells in culture film directed by Michael Abercrombie one of the father of quantitative cell imaging (University College London, 1955) is available here https://identity.wellcomecollection.org/works/z6h7jzv4

Comments

jdhl18.bsky.social•102 days ago

What was the video of light activated Rac that showed you could stimulate a change in direction of cell migration? I think it was you who showed it in a journal club many years ago?

guijacquemet.bsky.social•102 days ago

This is the original paper
https://www.nature.com/articles/nature08241#Sec20
And one of the SI video. But I have seen low magnification video where people control directional migration like a cat with a laser pointer

anhhle2702.bsky.social•102 days ago

Do we have an equivalent paper for RhoA and cell contraction?

guijacquemet.bsky.social•102 days ago

There are a few I believe, also with photoactivable small molecule inhibitors, but I am no longer on top of the literature with these topics anymore I'm afraid 😅

anhhle2702.bsky.social•102 days ago

Maybe @joachimgoedhart.bsky.social would know?

joachimgoedhart.bsky.social•102 days ago

There are several, our own paper was the easiest to find ;-)
https://elifesciences.org/articles/84364

lhinderling.bsky.social•102 days ago

Here is a movie locally activating RhoA from our paper https://doi.org/10.7554/eLife.90305.1
See fig. 4 for details. Our OptoLARG construct (pB3.0-optoLARG-mVenus-SspB-p2A-stargazin-mtq2-iLID) is adapted from this publication:
https://doi.org/10.1038/ncomms15817

anhhle2702.bsky.social•102 days ago

Ooh this is exactly what I have in mind. Thank you for sharing!

jdhl18.bsky.social•102 days ago

How did you find that so quickly! 👌

guijacquemet.bsky.social•102 days ago

I got these constructs nearly 15 years ago (😱😱) and I never got the chance to play with them

senger.bsky.social•102 days ago

Incredible, allready explored back in 2009...

senger.bsky.social•102 days ago

You have some nice movies from Orion Weiner and @lhinderling.bsky.social

lhinderling.bsky.social•102 days ago

@jpassmore.bsky.social has beautiful migration with fast HT1080?

Here are some slow & sticky REF52 from our lab:
https://bsky.app/profile/lhinderling.bsky.social/post/3laq4nlvaxs2o

jpassmore.bsky.social•102 days ago

Klaus Hahn has some classic movies of manually directing migration: https://tinyurl.com/49ftza3h. The Weiner lab use HL60 (v fast) continually illuminating one side: https://tinyurl.com/mryvy9m7 . Here is an example of our automatic guidance of HT1080 (slower) to specific paths, look out for a bioRxiv upload soon!🌀

jdhl18.bsky.social•102 days ago

Might help to slow that down a bit?

jpassmore.bsky.social•102 days ago

Sure! 🐌

jdhl18.bsky.social•102 days ago

📌

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