jcornlab.bsky.social
Genome editing, functional genomics, and cells figuring out how to eat themselves without dying. Professor of Genome Biology at ETH Zürich.
15 posts
291 followers
102 following
Getting Started
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I’m still learning Bluesky, so now tagging people I mis-tagged. Sorry! @charlesyeh.bsky.social @albertomarin.bsky.social @fedeteloni.bsky.social @gerlichlab.bsky.social
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If you’re interested in how loop-extruding cohesin drives the homology search during HR, check out also this new preprint from albertomarin.bsky.social in the Taekjip Ha lab (doi.org/10.1101/2025...)
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Curious about how cohesin guides homology search via loops & sister chromatid linkages? Check out this new preprint by fedeteloni.bsky.social & gerlichlab.bsky.social! 👉 tinyurl.com/DSBcohesin
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This project was driven by amazing graduate student charlesyeh.bsky.social, with lots of amazing collaborators over the years.
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Altogether, proximity is key and search normally stays around the DSB. When it comes choosing a donor, only these sequences are candidates.
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During genome editing, exogenous donor DNAs soak up homology search during HDR. Even if they have no sequence relationship to the cut site at all. They actually steal search from the genome!
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Only 3D-nearby those sequences that are searched (i.e., candidates) are chosen as HDR donors! Donors too far away are never seen and never used.
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Search is constrained by chromatin conformation. Check out the related preprints from fedeteloni.bsky.social
and albertomarin.bsky.social for a much deeper dive (see later in thread).
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We call this "RaPID-seq". It unveils a new dimension of DNA repair, telling us what is searched during HDR. In other words, what are the candidates?
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This project was led by
@matthiasmuhar.bsky.social, @jakobfarnung.bsky.social Martina Cernakova, and Raphael Hofmann.
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Thanks to amazing collaborators in the Schulman, Jinek, and Jessberger labs. And most of all to Jeff Bode's lab, who has been with this every step of the way. @micharapelab.bsky.social wrote a News and Views that explains our work far better than I ever could. www.nature.com/articles/d41...
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In Berkeley: www.darkcarnival.com