sarajweaver.bsky.social - Profile | ThreadSky | a Reddit-style client for Bluesky

The Gonen Lab at UCLA is seeking a Postdoctoral Associate. Join the team to engage in projects in membrane biophysics and biochemistry; membrane protein purification and expression experience required. Lab website:Apply online: tinyurl.com/y4y8uvbr cryoem.ucla.edu

submitted 1671 days ago • 0 comments

www.biorxiv.org/content/10.1... #teamtomo #cryoET

submitted 4 days ago • 1 comment

Join us at Amgen Structural Biology in SoCal! Work on #xray and #cryoEM. careers.amgen.com/en/job/thous...

submitted 3 days ago • 0 comments

Fast event-based electron counting for small-molecule structure determination by MicroED pubmed.ncbi.nlm.nih.gov/39982366/ #cryoEM

submitted 8 days ago • 0 comments

submitted 18 days ago • 0 comments

Niko Vlahakis et al.: Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination #CryoEM #MicroED #Nanocrystals @ucla...#IUCr https://journals.iucr.org/paper?S2052252524012132

submitted 20 days ago • 0 comments

Interested in #CryoEM structure refinement? Then this #webinar is for you: "Cryo-EM structure refinement with density-guided simulations in #GROMACS" 🗓️ 4 March 2025, 15:00 CET Registration & further info ➡️ bit.ly/41b6L7W

submitted 20 days ago • 0 comments

Nice - first CryoEM structure of ALK2 in complex with a ligand! #BMP6 #ACVR1

submitted 20 days ago • 0 comments

Mystery Protein Challenge Calling all structural biology enthusiasts and #cryoEM aficionados! Can you identify the protein shrouded in this 2D class average? Hint: This protein plays a vital role in the respiratory chain.

submitted 20 days ago • 2 comments

Are you doing cell #biology? Then, let me guess, you likely stained #actin! Our @jcb.org article reveals the structure of the popular actin probe F-tractin in complex with F-actin and introduces an optimized version for better imaging! A thread! 👀👇 rupress.org/jcb/article/...

submitted 19 days ago • 1 comment

Accounting for electron-beam-induced warping of molecular nanocrystals in MicroED structure determination pubmed.ncbi.nlm.nih.gov/39927752/ #cryoEM

submitted 19 days ago • 0 comments

(BioRxiv All) CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes from Azotobacter vinelandii: Single particle cryoelectron microscopy (cryoEM) and cryoelectron tomography (cryoET) are powerful methods for unveiling… http://dlvr.it/THnVMD #BioRxiv #MassSpecRSS

submitted 26 days ago • 0 comments

Complex Water Networks Visualized through 2.2-2.3 A Cryogenic Electron Microscopy of RNA pubmed.ncbi.nlm.nih.gov/39896454/ #cryoEM

submitted 26 days ago • 0 comments

I’m happy to share gapTrick, an AlphaFold2-based tool for characterising protein-protein complexes www.biorxiv.org/content/10.1...

submitted 25 days ago • 1 comment

Are PDB models biased in terms of how well they explain the underlying experimental data and are modeled? www.biorxiv.org/content/10.1...

submitted 77 days ago • 1 comment

Casual reminder that what many consider 'information' from #crystallography is actually a model. That is why structure factors must always be published, so that reviewers and anyone can see what lies behind the model. Without them, crystal structures are nothing more than 'pictures' in 3D.

submitted 93 days ago • 3 comments

Following a suggestion from @benecal.bsky.social I will refer to these threads as "skeetorials" and will use this gif logo. Today's skeetorial is about the NIH Director's New Innovator Award with three different heroes (all in government). 1/n

submitted 100 days ago • 10 comments

Who are some amazing cryoEM/cryoET, mass spec, and light microscopy scientists coming to San Diego for ASCB? I’m making a list for a potential pre-ASCB workshop at UCSD December 13th. Spread the word and let me know if you know a good candidate to speak at such an event!

submitted 99 days ago • 0 comments

🚨 Our story on the TSC:WIPI3 lysosomal-recruitment complex is now live in Science Advances. Equal-first co-authors @luptoncj.bsky.social & Laura D'Andrea. Work lead by @drellisdon.bsky.social. Funding from @deptofdefense.bsky.social & Australian Research Council. www.science.org/doi/10.1126/...

submitted 102 days ago • 5 comments

Great to see our work on the #cryoem structure of the human TSC:WIPI3 lysosomal recruitment complex! Awesome effort from co-authors @charlesbj.bsky.social and Laura D'Andrea with @drellisdon.bsky.social doing the heavy lifting leading the project. www.science.org/doi/10.1126/...

submitted 102 days ago • 0 comments

Question for the #CryoEM crowd: does anyone happen to recognize this lovely ~10-15nm diameter protein complex we stumbled across while imaging slime mold protein lysate (as one does)?

submitted 102 days ago • 5 comments

👇 Did you know one can use pulsed lasers not only to increase contrast in #cryoEM images, but also to reduce preferred particle orientation, by melting & re-vitrifying samples in a controlled way? ❄️🔬

submitted 101 days ago • 0 comments

Congratulations 🎉 to the doctoral student-mentor pairs named HHMI Gilliam Fellows including @cobarnes27.bsky.social @adonisrubio.bsky.social news.stanford.edu/stories/2024...

submitted 100 days ago • 2 comments

This is amazing! @shervinnia.bsky.social resurrected our cryoEM-papers bot. At it's peak we had over 12k followers on Twitter, but the bot became defunct by a silly Musk policy (and it all went downhill from there). If you want to keep up with #cryoEM papers, follow: cryoempapers.bsky.social

submitted 103 days ago • 1 comment

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

submitted 114 days ago • 72 comments

Prof. Mikhail Shapiro is giving a monthly course on "Synthetic biology for physicists". The 2nd lecture will take place this Friday 22/11 from 10:30 to 12:30, and will be entitled "What can proteins do for physical remote control?". ⬇️

submitted 103 days ago • 1 comment

Anyone experienced with X. laevis husbandry? We are having a terrible time with poorly developed legs, and I don't know what to try next to fix it. I have never had this kind of issue with raising tadpoles to froglets before. It's stressing me out! 🐸 🧪 @jbwallingford.bsky.social ?

submitted 129 days ago • 6 comments

Been thinking about creating a collection of good protein structure figures, as inspiration for my own work. #1 www.nature.com/articles/s41...

submitted 365 days ago • 5 comments

CryoCycle your grids: Plunge vitrifying and reusing clipped grids to advance cryoEM democratization https://www.biorxiv.org/content/10.1101/2024.01.23.576763v1

submitted 404 days ago • 0 comments

blik: an extensible napari plugin for cryo-ET data visualisation, annotation and analysis https://www.biorxiv.org/content/10.1101/2023.12.05.570263v1

submitted 451 days ago • 0 comments

Fantastic work!

submitted 446 days ago • 1 comment

Not sure how many SerialEM users in #cryoEM and #cryoET are already here, but: SerialEM 4.1 was just released. It comes with many useful new features and script commands, thanks to the tireless work of David Mastronarde bio3d.colorado.edu/ftp/SerialEM...

submitted 446 days ago • 0 comments

DeepMainmast: integrated protocol of protein structure modeling for cryo-EM with deep learning and structure prediction - Nature Methods www.nature.com/artic... --- #proteomics #prot-paper

submitted 451 days ago • 0 comments

Yay, the all-new MRC-LMB 2023 #cryoEM course lectures are now online! Happy learning. 🥳 www.youtube.com/playlist?lis...

submitted 451 days ago • 0 comments

This was missed due to major live events happening around the same time, but we have released some of our high-resolution (2 Å) and rapid screening #cryoEM maps & models of the human CAK bound to small molecules. Paper still under review - more to follow. www.ebi.ac.uk/emdb/search/...

submitted 493 days ago • 0 comments

Cryo-electron tomography reveals the structural diversity of cardiac proteins in their cellular context https://www.biorxiv.org/content/10.1101/2023.10.26.564098v1

submitted 493 days ago • 0 comments

Meet the PyShell!🥧🐚 Diatom pyrenoids fix up to 20% of the Earth's #CO2🌏. We discovered that their Rubisco is encased in a tight protein lattice that is essential for efficient carbon fixation and cell growth🌱 Super excited to share our new preprint📜: www.biorxiv.org/content/10.1... 🌾🧪 🧶🧬 ❄️🔬

submitted 493 days ago • 5 comments

Given small molecules locally resemble amino acid sidechains, we (Artem Gazizov, Anna Lian, Casper Goverde, Nicholas Polizzi) wondered if AF2 could be used to predict binding sites, when provided with a structure and a string of AAs. (1/10) *[looking for PhD opportunities] 🤓

submitted 500 days ago • 3 comments

And our new #DynaMight paper is out on @biorxivpreprint.bsky.social! 🥳 This is #RELION5's answer to modelling molecular flexibility by the amazing Johannes Schwab. He also shows that model bias can be nasty when modelling molecular flexibility with many parameters. www.biorxiv.org/content/10.1...

submitted 501 days ago • 1 comment

Biochem/structural bio colleagues, does anyone have experience with this technology? We are looking an alternative to mass photometry that covers a wider range of MWs (from peptides to large multi-subunits complexes) www.fidabio.com/product

submitted 501 days ago • 2 comments